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Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip

Plant Cell Reports volume 22, pages 859–863 (2004)Cite this article

Abstract

In this research we eliminated chrysanthemum stunt viroid (CSVd) from a highly infected chrysanthemum cultivar using a newly established method. ‘Piato’ is one of the most difficult cultivars in which to obtain CSVd-free plants by conventional methods. Leaf primordium-free shoot apical meristems (LP-free SAMs) of ‘Piato’ plants were dissected and attached to CSVd-free chrysanthemum or cabbage root tips. As shown by nested-PCR, CSVd was not detected in some shoots regenerated on both types of root tip. The production rates of CSVd-free plants using chrysanthemum and cabbage root tips were 14% and 3%, respectively. Regeneration of plants from LP-free SAMs of chrysanthemum plants by attaching these SAMs to root tips is an efficient method of generating CSVd-free chrysanthemum plants.

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Affiliations

  1. Laboratory of Vegetable and Ornamental Horticulture, Department of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606–8502, Kyoto, Japan

    M. Hosokawa, A. Otake, K. Ohishi, E. Ueda, T. Hayashi & S. Yazawa

Authors
  1. M. Hosokawa
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  2. A. Otake
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  3. K. Ohishi
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  4. E. Ueda
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  5. T. Hayashi
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  6. S. Yazawa
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Corresponding author

Correspondence to M. Hosokawa.

Additional information

Communicated by K.K. Kamo

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Hosokawa, M., Otake, A., Ohishi, K. et al. Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip. Plant Cell Rep 22, 859–863 (2004). https://doi.org/10.1007/s00299-004-0770-6

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  • DOI: https://doi.org/10.1007/s00299-004-0770-6

Keywords

  • Leaf primordium-free shoot apical meristem
  • Root tip
  • Chrysanthemum
  • Chrysanthemum stunt viroid
  • CSVd-free