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Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos

  • Cell Biology and Morphogenesis
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Abstract

Embryogenic culture was initiated from mature zygotic embryos of Panax ginseng. Multiple somatic embryos formed and proliferated on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (2.26 μM) and kinetin (0.046 μM). Mature as well as immature somatic embryos grew into plantlets lacking roots on the same media. Histomorphological analysis of somatic embryos treated with abscisic acid (ABA) and polyethylene glycol (PEG 4000) showed a slight improvement in the root meristem organization of torpedo-stage embryos (embryos were more compact and their cells exhibited a lower degree of vacuolation). Shoot regeneration of non-treated somatic embryos was 31% while that for somatic embryos treated with PEG 4000 and ABA was 70%. Moreover, 75% of plants regenerated from PEG- and ABA-treated embryos formed roots while plants from non-treated embryos did not form roots.

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Fig. 1A–F

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Abbreviations

ABA :

(±)-Abscisic acid

BAP :

N 6-Benzyladenine

2,4-D :

2,4-Dichlorophenoxyacetic acid

GA 3 :

Gibberellic acid

Kin :

Kinetin

MS :

Murashige and Skoog medium

PEG 4000 :

Polyethylene glycol 4000

PGR :

Plant growth regulators

References

  • Arnold S von, Hakman I (1988) Regulation of somatic embryo development in Picea abies by abscisic acid (ABA). J Plant Physiol 132:164–169

    Google Scholar 

  • Arya S, Arya ID, Eriksson T (1993) Rapid multiplication of adventitious somatic embryos of Panax ginseng. Plant Cell Tissue Organ Cult 34:157–162

    CAS  Google Scholar 

  • Attree SM, Moore D, Sawhney VR, Fowke LC (1991) Enhanced maturation and desiccation tolerance of white spruce [Picea glauca (Moench.) Voss] somatic embryos: effects of a non-plasmolysing water stress and abscisic acid. Ann Bot 68:519–525

    Google Scholar 

  • Bozhkov PV, von Arnold S (1998) Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiol Plant 104:211–224

    Article  CAS  Google Scholar 

  • Bruneton J (1995) Pharmacognosy, phytochemistry, medicinal plants. Lavoisier, Paris, pp 563–567

  • Butenko RG, Brushwitzky IV, Stepyan LI (1968) Organogenesis and somatic embryogenesis in the tissue culture of Panax ginseng C.A. Meyer. Bot Zh 7:906–913

    Google Scholar 

  • Čeliárová E, Rychlová M, Vranová E (1992) Histological characterization of in vitro regenerated structures of Panax ginseng. Plant Cell Tissue Organ Cult 30:165–170

    Google Scholar 

  • Chang WC, Hsing YE (1980) Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C.A. Meyer). Theor Appl Genet 57:133–135

    Google Scholar 

  • Choi KT (1988) Panax ginseng C.A. Meyer: micropropagation and the in vitro production of saponins. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants. I. Springer, Berlin Heidelberg New York, pp 484–499

  • Choi YE, Soh WY (1996) Effect of plumule and radicle on somatic embryogenesis in the cultures of ginseng zygotic embryos. Plant Cell Tissue Organ Cult 45:137–143

    Google Scholar 

  • Choi YE, Yang DC, Kim HS, Choi KT (1997) Distribution and changes of reserve materials in cotyledon cells of Panax ginseng related to direct somatic embryogenesis and germination. Plant Cell Rep 16:841–846

    Article  CAS  Google Scholar 

  • Choi YE, Yang DC, Park JC, Soh WY, Choi KT (1998) Regenerative ability of somatic single and multiple embryos from cotyledons of Korean ginseng on hormone-free medium. Plant Cell Rep 17:544–551

    Article  CAS  Google Scholar 

  • Debergh P, Aitken-Christie J, Cohen D, Grout B, Von Arnold S, Zimmermann R, Ziv M (1992) Reconsideration of the term “vitrification” as used in micropropagation. Plant Cell Tissue Organ Cult 30:135–160

    Google Scholar 

  • Hong SK (1982) Ginseng cultivation. In: Atal CK, Kapur BM (eds) Cultivation and utilization of medicinal plants. Regional Research Laboratory (CSIR), Jammu-Tawi, New Delhi, p 877

  • Johansen DA (1940) Plant microtechnique. McGraw-Hill, New York

  • Joshi A, Teng WL (2000) Cryopreservation of Panax ginseng cells. Plant Cell Rep 19:971–977

    Google Scholar 

  • Kevers C, Gaspar T, Dommes J (2002) The beneficial role of different auxins and polyamines at successive stages of somatic embryo formation and development of Panax ginseng in vitro. Plant Cell Tissue Organ Cult 70:181–188

    Article  CAS  Google Scholar 

  • Kongbangkerd A, Wawrosch C (2003) Improved shoot regeneration from nodules of Charybdis numidica in a temporary immersion system. J Hortic Sci Biotechnol 78:650–655

    CAS  Google Scholar 

  • Langhansová L, Nepovím A, Maršík P, Vaněk T (2002) Saponin production from in vitro cell and root cultures of Panax ginseng C. A. Meyer. Plant biotechnology: 2002 and beyond. The 10th IAPTC&B Congress, Orlando, USA, p 82-A

    Google Scholar 

  • Lee HS, Liu JR, Yang SG, Lee YH (1990) In vitro flowering of plantlets regenerated from zygotic embryo-derived somatic embryos of ginseng. HortScience 25:1652–1654

    Google Scholar 

  • Linossier L, Veisseire P, Cailloux F, Coudret A (1997) Effects of abscisic acid and high concentrations of PEG on Hevea brasiliensis somatic embryos development. Plant Sci 124:183–191

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Poláčková D, Beneš K (1975) The staining of chromosomes and nuclei in squashes of root tip with aluminium lake of nuclear fast red. Biol Plant 17:374–375

    Google Scholar 

  • Sagare AP, Lee YL, Lin TC, Chen CC, Tsay HS (2000) Cytokinin-induced somatic embryogenesis and plant regeneration in Corydalis yanhusuo (Fumariaceae)—a medicinal plant. Plant Sci 160:139–147

    Article  CAS  PubMed  Google Scholar 

  • Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204

    CAS  Google Scholar 

  • Seitz U, Reinhard E (1987) Growth and ginsenoside patterns of cryopreserved Panax ginseng cell cultures. J Plant Physiol 131:215–223

    CAS  Google Scholar 

  • Shoyama Y, Matsushita H, Zhu XX, Kishira H (1995) Somatic embryogenesis in Ginseng (Panax Species). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 31. Somatic embryogenesis and synthetic seed. II. Springer, Berlin Heidelberg New York, pp 343–356

  • Stasolla C, Kong L, Yeung EC, Thorpe TA (2002) Maturation of somatic embryos in conifers: morphogenesis, physiology, biochemistry and molecular biology. In Vitro Cell Dev Biol Plant 38:93–105

    Article  CAS  Google Scholar 

  • Stasolla C, van Zyl L, Egertsdotter U, Craig D, Liu W, Sederoff RR (2003) The effects of polyethylene glycol on gene expression of developing white spruce somatic embryos. Plant Physiol 131:49–60

    Article  CAS  PubMed  Google Scholar 

  • Tang W (2000) High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng. Plant Cell Rep 19:727–732

    Article  CAS  Google Scholar 

  • Tirajoh A, Kyung TS, Punja ZK (1998) Somatic embryogenesis and plantlet regeneration in American ginseng (Panax quinquefolium L.) In Vitro Cell Dev Biol Plant 34:203–211

  • Walker DR, Parrott WA (2001) Effect of polyethylene glycol and sugar alcohols on soybean somatic embryo germination and conversion. Plant Cell Tissue Organ Cult 64:55–62

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by GACR grant number 521/02/P064, COST project 843.10 and research project Z4 055 905.

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Authors and Affiliations

  1. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of Czech Republic, Flemingovo nám. 2, 166 10, Prague 6, Czech Republic

    L. Langhansová & T. Vaněk

  2. Department of Plant Physiology, Faculty of Science, Charles University, Viničná 5, 128 44, Prague 2, Czech Republic

    H. Konrádová

Authors
  1. L. Langhansová
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  2. H. Konrádová
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  3. T. Vaněk
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Correspondence to T. Vaněk.

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Communicated by H. van Onckelen

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Langhansová, L., Konrádová, H. & Vaněk, T. Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos. Plant Cell Rep 22, 725–730 (2004). https://doi.org/10.1007/s00299-003-0750-2

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  • DOI: https://doi.org/10.1007/s00299-003-0750-2

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