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Effect of light on the organogenic ability of garlic roots using a one-step in vitro system

  • Cell Biology and Morphogenesis
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Abstract

A simple and efficient garlic in vitro shoot regeneration protocol has been developed. This system uses axenic root tips cultivated from the beginning in the presence of light and does not require any change or refreshing of the original medium during the entire process. The application of light from the beginning of the culture process did not affect the callus formation rate but did significantly improve the explant regeneration ability. In a 2-month period it was possible to obtain up to 250 shoots per gram of callus.

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Abbreviations

BAP::

6-Benzylaminopurine

2,4-D::

2,4-Dichlorophenoxyacetic acid

NAA::

α-Naphthaleneacetic acid

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Acknowledgements

This work was partially funded by a national project (INIA, no. RF00-014) and a EU project (no. QLK1-CT-1999-00498).

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Authors and Affiliations

  1. Departamento de Microbiología, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales C6, 14071, Cordoba, Spain

    J. Garrido-Gala, J. Martín & X. Barandiaran

  2. Allium Laboratorios S.L., c/o Gabriel Ramos Bejarano, puerta I, parc. 110D, Polígono Industrial “Las Quemadas”, 14014, Cordoba, Spain

    N. Martín-Urdíroz

Authors
  1. N. Martín-Urdíroz
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  2. J. Garrido-Gala
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  3. J. Martín
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  4. X. Barandiaran
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Corresponding author

Correspondence to N. Martín-Urdíroz.

Additional information

Communicated by L. Peña

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Martín-Urdíroz, N., Garrido-Gala, J., Martín, J. et al. Effect of light on the organogenic ability of garlic roots using a one-step in vitro system. Plant Cell Rep 22, 721–724 (2004). https://doi.org/10.1007/s00299-003-0744-0

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  • DOI: https://doi.org/10.1007/s00299-003-0744-0

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