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Plant Cell Reports

, Volume 22, Issue 8, pp 608–614 | Cite as

Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea

  • E. Ait BarkaEmail author
  • P. Eullaffroy
  • C. Clément
  • G. Vernet
Physiology and Biochemistry

Abstract

We evaluated the potential of chitosan both to stimulate plant development and to induce protection from Botrytis cinerea in Vitis vinifera L. plantlets. The presence of 1.75% (v/v) chitogel in the culture medium was the optimal concentration for in vitro grapevine plantlet growth, as determined by measurements on enhancement of root and shoot biomass. Photosynthesis and related parameters were also stimulated in chitogel-treated plantlets. Chitogel reduced the development of Botrytis cinerea and induced cytological alterations to the pathogen. When challenged with the fungus, a significant decrease in disease incidence was observed in plants growing on medium supplemented with chitogel. Furthermore, exogenous foliar applications of chitogel to plantlets growing on chitogel-free medium sensitized them so as to be protected against Botrytis cinerea attack. Our results indicate that chitogel can be used in the vineyard as a means to attain protection against Botrytis cinerea and that its application may counteract the wide use of chemical pesticides.

Keywords

Botrytis cinerea Chitogel Bioorganic substance Vitis vinifera L. 

Notes

Acknowledgements

The present study was realized at Université de Reims Champagne-Ardenne, and was in part supported by Europol’Agro, Reims, France. The authors thank B. Gallois from Ecobulle Ltd (Cormontreuil, France) for providing the chitogel.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • E. Ait Barka
    • 1
    Email author
  • P. Eullaffroy
    • 2
  • C. Clément
    • 1
  • G. Vernet
    • 2
  1. 1.UFR Sciences, URVVC, UPRES EA 2069, Laboratoire de Biologie et Physiologie VégétalesUniversité de Reims Champagne-ArdenneReims Cedex 2France
  2. 2.UFR Sciences, URVVC, UPRES EA 2069, Laboratoire d’ Eco-ToxicologieUniversité de Reims Champagne-ArdenneReims Cedex 2France

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