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Interactions of phytochromes A, B1 and B2 in light-induced competence for adventitious shoot formation in hypocotyl of tomato (Solanum lycopersicum L.)

  • Physiology and Biochemistry
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Abstract

The interactions of phytochrome A (phyA), phytochrome B1 (phyB1) and phytochrome B2 (phyB2) in light-dependent shoot regeneration from the hypocotyl of tomato was analysed using all eight possible homozygous allelic combinations of the null mutants. The donor plants were pre-grown either in the dark or under red or far-red light for 8 days after sowing; thereafter hypocotyl segments (apical, middle and basal portions) were transferred onto hormone-free medium for culture under different light qualities. Etiolated apical segments cultured in vitro under white light showed a very high frequency of regeneration for all of the genotypes tested besides phyB1phyB2, phyAphyB1 and phyAphyB1phyB2 mutants. Evidence is provided of a specific interference of phyB2 with phyA-mediated HIR to far-red and blue light in etiolated explants. Pre-treatment of donor plants by growth under red light enhanced the competence of phyB1phyB2, phyAphyB1 and phyAphyB1phyB2 mutants for shoot regeneration, whereas pre-irradiation with far-red light enhanced the frequency of regeneration only in the phyAphyB1 mutant. Multiple phytochromes are involved in red light- and far-red light-dependent acquisition of competence for shoot regeneration. The position of the segments along the hypocotyl influenced the role of the various phytochromes and the interactions between them. The culture of competent hypocotyl segments under red, far-red or blue light reduced the frequency of explants forming shoots compared to those cultured under white light, with different genotypes having different response patterns.

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Abbreviations

HIR::

High irradiance response

LFR::

Low fluence response

Pfr::

Far-red absorbing form of phytochrome

phyA::

Phytochrome A

phyB1::

Phytochrome B1

phyB2::

Phytochrome B2

phyA(B1, B2)::

Phytochrome mutant deficient in phyA (B1, B2)

phyAphyB1(B1B2,AB2)::

Double phytochrome mutant deficient in phyA and phyB1(B1, B2)

phyAphyB1phyB2::

Triple mutant deficient in phyA, phyB1 and phyB2

VLFR::

Very low fluence response

WT::

Wild-type tomato

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Acknowledgements

We thank Dr. R. Kendrick for his generous gift of tomato mutant seeds, Alberto Manetti for excellent technical assistance. This work was supported by MIUR, progetti di interesse nazionale.

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  1. Dipartimento di Biologia delle Piante Agrarie, Università di Pisa, Viale delle Piagge 23, 56124, Pisa, Italy

    B. Lercari & L. Bertram

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  1. B. Lercari
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  2. L. Bertram
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Correspondence to B. Lercari.

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Communicated by R. Reski

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Lercari, B., Bertram, L. Interactions of phytochromes A, B1 and B2 in light-induced competence for adventitious shoot formation in hypocotyl of tomato (Solanum lycopersicum L.). Plant Cell Rep 22, 523–531 (2004). https://doi.org/10.1007/s00299-003-0725-3

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  • DOI: https://doi.org/10.1007/s00299-003-0725-3

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