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Induced androgenesis in tomato (Lycopersicon esculentum Mill.). III. Characterization of the regenerants

  • Cell Biology and Morphogenesis
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Abstract

We present data on the morphological, cytological, biochemical and genetic characteristics of tomato regenerants obtained through anther culture. As a result of induced androgenesis, more than 6,000 rooted regenerants were developed that differed both from the donor plants and among each other with respect to habitus and leaf, flower and inflorescence morphology. Cytological analysis revealed a great variability in chromosome number in the cells of the regenerated plants. While most of the regenerants were mixoploid, the majority of the cells had a haploid chromosome number. R1 and R2 progenies were tested for their resistance to Clavibacter michiganense subsp. michiganense (Cmm 7). Some of the regenerants were resistant to the pathogen. A biochemical analysis of fruit from R3 and R4 plants showed a higher content of dry matter, sugars and vitamin C in the regenerant plants obtained from the hybrids than in those from the cultivars and control plants. The values of the parameters of hybrid regenerants grown in the greenhouse were about 1.5-fold higher than those of the hybrid regenerants grown in the field, and this trend is clearly expressed in all of the hybrid regenerants. The results obtained suggest that induced androgenesis and gametoclonal variation may be used as an additional tool to create a large range of new forms. The application of the latter in breeding programs would accelerate the development of tomato lines and varieties that would be more productive, disease-resistant, highly nutritive and flavour-acceptable.

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Fig. 1
Fig. 2A–C
Fig. 3A, B

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Abbreviations

BAP :

N6-Benzylaminopurine

Cmm :

Clavibacter michiganense subsp. michiganense

cfu :

Colony-forming units

GA 3 :

Gibberellic acid

IBA :

Indole-3-butyric acid

ms :

Male sterility

PDA :

Potato dextrose agar

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Authors and Affiliations

  1. Institute of Genetics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    N. A. Zagorska, L. A. Shtereva, M. M. Kruleva, V. G. Sotirova, D. L. Baralieva & B. D. Dimitrov

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  1. N. A. Zagorska
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  2. L. A. Shtereva
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  3. M. M. Kruleva
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  4. V. G. Sotirova
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  5. D. L. Baralieva
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  6. B. D. Dimitrov
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Correspondence to N. A. Zagorska.

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Communicated by H. Lörz

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Zagorska, N.A., Shtereva, L.A., Kruleva, M.M. et al. Induced androgenesis in tomato (Lycopersicon esculentum Mill.). III. Characterization of the regenerants. Plant Cell Rep 22, 449–456 (2004). https://doi.org/10.1007/s00299-003-0720-8

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  • DOI: https://doi.org/10.1007/s00299-003-0720-8

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