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Taxonomic discrimination of higher plants by pyrolysis mass spectrometry

  • Physiology and Biochemistry
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Abstract

Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R. multiflora var. platyphylla, Sedum kamtschaticum, S. takesimense, S. sarmentosum, Hepatica insularis, and H. asiatica) were subjected to PyMS for spectral fingerprinting. Principal component analysis of PyMS data was not able to discriminate these plants in discrete clusters. However, canonical variate analysis of PyMS data separated these plants from one another. A hierarchical dendrogram based on canonical variate analysis was in agreement with the known taxonomy of the plants at the variety level. These results indicate that PyMS is able to discriminate higher plants based on taxonomic classification at the family, genus, species, and variety level.

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Fig. 1A, B
Fig. 2
Fig. 3A, B

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Abbreviations

ANNs :

Artificial neural networks

CVA :

Canonical variate analysis

GC/MS :

Gas chromatography/mass spectrometry

PCA :

Principal component analysis

PyMS :

Pyrolysis mass spectrometry

UPGMA :

Unweighted pair group method with arithmetic mean

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Acknowledgements

This work was supported by a grant (no. M10104000234-01J000-10710) to JRL from the National Research Laboratory Program, a grant (no. BDM 0100211) to JRL from the Strategic National R&D Program through the Genetic Resources and Information Network Center, and a grant to JRL from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center of the Kyung Hee University funded by the Korean Ministry of Science and Technology.

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Authors and Affiliations

  1. Laboratory of Plant Genomics Services and Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoun-dong, Yuseong-gu, 305-333, Daejeon, Korea

    S. W. Kim, S. H. Ban & J. R. Liu

  2. Laboratory of Functional Genomics for Plant Secondary Metabolism (National Research Laboratory), Eugentech Inc., 52 Eoun-dong, Yuseong-gu, 305-333, Daejeon, Korea

    H. J. Chung, D. W. Choi & P. S. Choi

  3. Department of Biological Science, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yuseong-gu, 305-701, Daejeon, Korea

    O. J. Yoo

Authors
  1. S. W. Kim
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  2. S. H. Ban
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  3. H. J. Chung
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  4. D. W. Choi
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  5. P. S. Choi
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  6. O. J. Yoo
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  7. J. R. Liu
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Corresponding author

Correspondence to J. R. Liu.

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Communicated by I.S. Chung

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Kim, S.W., Ban, S.H., Chung, H.J. et al. Taxonomic discrimination of higher plants by pyrolysis mass spectrometry. Plant Cell Rep 22, 519–522 (2004). https://doi.org/10.1007/s00299-003-0714-6

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  • DOI: https://doi.org/10.1007/s00299-003-0714-6

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