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Production of transgenic lily plants by Agrobacterium-mediated transformation

  • Cell Biology and Morphogenesis
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Abstract

A system for the production of transgenic plants was developed for the Oriental hybrid lily, Lilium cv. Acapulco, by Agrobacterium-mediated genetic transformation. Filament-derived calli were co-cultivated with A. tumefaciens strain EHA101/pIG121Hm, which harbored a binary vector carrying the neomycin phosphotransferase II, hygromycin phosphotransferase, and intron-containing β-glucuronidase genes in the T-DNA region. Six hygromycin-resistant (Hygr) culture lines were obtained from 200 calli by scratching them with sandpaper prior to inoculation and using NH4NO3-free medium for co-cultivation and a hygromycin-containing regeneration medium for selection. Hygr culture lines regenerated shoots, which developed into plantlets following transfer to a plant growth regulator-free medium. All of these plantlets were verified to be transgenic by GUS histochemical assay and inverse PCR analysis.

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Fig. 1A–H.
Fig. 2A, B.

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Abbreviations

AS :

Acetosyringone (3,5-dimethoxy-4-hydroxy-acetophenone)

BA :

Benzyladenine

CaMV :

Cauliflower mosaic virus

GUS :

β-Glucuronidase

HPT :

Hygromycin phosphotransferase

Hyg r :

Hygromycin-resistant

NOS :

Nopaline synthase

NPTII :

Neomycin phosphotransferase II

PGR :

Plant growth regulator

PIC :

Picloram (4-amino-3,5,6-trichloropicolinic acid)

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Authors and Affiliations

  1. Niigata Agricultural Research Institute, 857 Nagakura, 940-0826, Nagaoka, Niigata, Japan

    Y. Hoshi, M. Kondo & H. Kobayashi

  2. Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Niigata, Japan

    S. Mori, Y. Adachi & M. Nakano

Authors
  1. Y. Hoshi
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  2. M. Kondo
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  3. S. Mori
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  4. Y. Adachi
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  5. M. Nakano
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  6. H. Kobayashi
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Correspondence to Y. Hoshi.

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Communicated by H. Ebinuma

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Hoshi, Y., Kondo, M., Mori, S. et al. Production of transgenic lily plants by Agrobacterium-mediated transformation. Plant Cell Rep 22, 359–364 (2004). https://doi.org/10.1007/s00299-003-0700-z

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  • DOI: https://doi.org/10.1007/s00299-003-0700-z

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