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Thermoinduction of genes encoding the enzymes of gibberellin biosynthesis and a putative negative regulator of gibberellin signal transduction in Eustoma grandiflorum

  • Genetics and Genomics
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Abstract

Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3β-hydroxylase, were examined using two culitvars that show different responses to vernalization. The three genes were induced in a vernalization- and a cultivar-dependent manner. EgSPY, a putative negative regulator of GA signal transduction, was also induced during the vernalization period. The results suggest that the expression of the genes encoding GAs biosynthesis is regulated by vernalization. We postulate that EgSPY functions as a negative regulator of GA signal transduction during vernalization, inhibiting adventitious shoot elongation during vernalization.

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Author notes

  1. M. Mino

    Present address: Graduate School of Agriculture, Kyoto Prefectural University, 1–5 Hangi-Chou, Shimogamo, Sakyo-Ku, 606-8522, Kyoto, Japan

  2. M. Oka

    Present address: Faculty of Agriculture, Tottori University, 680-8553, Tottori, Japan

  3. Y. Tasaka

    Present address: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, 062-8517, Sapporo, Japan

Authors and Affiliations

  1. Research Institute for Biological Sciences, Okayama, Jobo-gun, Kayo-chou, 716-1241, Okayama, Japan

    M. Mino, M. Oka, Y. Tasaka & M. Iwabuchi

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  1. M. Mino
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  2. M. Oka
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  3. Y. Tasaka
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  4. M. Iwabuchi
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Correspondence to M. Mino.

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Communicated by K.K. Kamo

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Mino, M., Oka, M., Tasaka, Y. et al. Thermoinduction of genes encoding the enzymes of gibberellin biosynthesis and a putative negative regulator of gibberellin signal transduction in Eustoma grandiflorum . Plant Cell Rep 22, 159–165 (2003). https://doi.org/10.1007/s00299-003-0672-z

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  • DOI: https://doi.org/10.1007/s00299-003-0672-z

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