Abstract
Gastrodia antifungal proteins (GAFPs) are a group of mannose-binding lectins purified from Gastrodia elata that show strong resistance against a wide spectrum of fungi. The GAFP-2 promoter was analyzed for its ability to control the expression of the reporter gene, β-glucuronidase (GUS) in transgenic tobacco plants. The GUS assays revealed that the GAFP-2 promoter is expressed in a tissue-specific manner, which mainly expressed in the vascular cells. The highest GUS activity was observed in roots, followed by stems. GAFP-2-GUS expression was strongly induced by the fungus Trichoderma viride and by the plant stress regulators, salicylic acid and jasmonic acid in the stably transformed tobacco plants. The −537 region of the GAFP-2 promoter was sufficient for its tissue-specific and inducible expression of the promoter.
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Acknowledgements
We thank Dr. Shunxing Guo for the kind gift of Gastrodia elata and Dr. Xihua Du for assistance with the sections and photos. We also thank Dr. Chengcai Chu for the spectrophotometer and Prof. Guenter Kahl and Dr. Jianru Zuo for critical reading of this manuscript. This study was supported by the National Natural Science Foundation of China (grant No. 30270746).
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Communicated by H.S. Judelson
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Sa, Q., Wang, Y., Li, W. et al. The promoter of an antifungal protein gene from Gastrodia elata confers tissue-specific and fungus-inducible expression patterns and responds to both salicylic acid and jasmonic acid. Plant Cell Rep 22, 79–84 (2003). https://doi.org/10.1007/s00299-003-0664-z
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DOI: https://doi.org/10.1007/s00299-003-0664-z