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An effective method for axillary bud culture and RAPD analysis of cloned plants in tetraploid black locust

  • Cell Biology and Morphogenesis
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Abstract

An efficient micropropagation technique by axillary bud multiplication was established for cloning tetraploid black locust tree (Robinia pseudoacacia L.). The result showed that the optimal medium for shoot multiplication and elongation was Murashige and Skoog (MS) medium supplemented with 0.5 mg/l 6-benzylaminopurine in combination with 0.5 mg/l kinetin and 0.1 mg/l 1-naphthaleneacetic acid. The best medium for rooting was half-strength MS medium with 0.25 mg/l indole-3-butyric acid. In the present report, we examined the genetic fidelity of the micropropagated plants by the randomly amplified polymorphic DNA (RAPD) method with 25 primers. The cloned plants of tetraploid black locust showed complete stability.

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Fig. 1A–F.
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Abbreviations

BAP :

6-Benzylaminopurine

GA 3 :

Gibberellic acid

IBA :

Indole-3-butyric acid

KIN :

Kinetin

MS :

Murashige and Skoog medium

NAA :

1-Naphthaleneacetic acid

PCR:

Polymerase chain reaction

RAPD :

Randomly amplified polymorphic DNA

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Acknowledgements

Our special thanks are given to our co-worker Mrs. Fang-fang Li for her technical assistance. The Biotechnology of Pasture Plant Program is funded by the Key Project of the Chinese Academy of Sciences (KSCX1-08).

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Author notes

  1. H. J. Li

    Present address: Department of Plant Pathology, Washington State University, PO Box 646430, Pullman, Washington, WA 99164-6430, USA

Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China

    Q. Y. Shu, G. S. Liu, D. M. Qi, C. C. Chu, J. Liu & H. J. Li

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  1. Q. Y. Shu
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  2. G. S. Liu
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  3. D. M. Qi
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  4. C. C. Chu
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  5. J. Liu
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  6. H. J. Li
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Corresponding author

Correspondence to G. S. Liu.

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Communicated by M. Beckert

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Shu, Q.Y., Liu, G.S., Qi, D.M. et al. An effective method for axillary bud culture and RAPD analysis of cloned plants in tetraploid black locust. Plant Cell Rep 22, 175–180 (2003). https://doi.org/10.1007/s00299-003-0661-2

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  • DOI: https://doi.org/10.1007/s00299-003-0661-2

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