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Chilling stability of microtubules in root-tip cells of cucumber

  • Cell Biology and Morphogenesis
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Abstract

The chilling stability of microtubules was investigated in root-tip cells of cucumber (Cucumis sativus L.) using immunofluorescence labeling and a confocal laser scanning microscope. After low temperature (4°C) treatment for 5 h, we found not only chilling-stable cortical microtubules under the plasma membrane of the root-tip cells, but also chilling-stable punctate microtubules in the cytoplasm, which might be the microtubules associated with organelles. 6-Dimethylaminopurine (6-DMAP), an inhibitor of protein kinase, reduced the chilling stability of the cortical microtubules in the cells. When the root-tip cells were restored to normal growth temperature (28°C), the recovery of the microtubules seemed to be closely associated with the microtubules still remaining in these cells after chilling treatment.

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Fig. 1A–D.
Fig. 2A–C.
Fig. 3A–C.
Fig. 4A, B.
Fig. 5A, B.

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Abbreviations

6-DMAP :

6-Dimethylaminopurine

DMSO :

Dimethyl sulfoxide

FITC :

Fluorescein isothiocyanate

MTOCs :

Microtubule-organizing centers

PPB :

Pre-prophase band

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Acknowledgements

This study was supported by Hebei Province Science Foundation to Hong Zhang and National Natural Science Foundation of China (No. 30170458 and 30070049) to Yan Li.

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Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing , P.R. China

    J.-L. Zhao, X.-J. Li & Y. Li

  2. Department of Horticulture, Hebei Agricultural University, 077001, Baoding , P.R. China

    J.-L. Zhao & H. Zhang

Authors
  1. J.-L. Zhao
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  2. X.-J. Li
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  3. H. Zhang
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  4. Y. Li
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Correspondence to Y. Li.

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Communicated by H. Wang

J.-L. Zhao and X.-J. Li are considered joint first authors

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Zhao, JL., Li, XJ., Zhang, H. et al. Chilling stability of microtubules in root-tip cells of cucumber. Plant Cell Rep 22, 32–37 (2003). https://doi.org/10.1007/s00299-003-0656-z

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  • DOI: https://doi.org/10.1007/s00299-003-0656-z

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