Plant Cell Reports

, Volume 22, Issue 1, pp 59–63 | Cite as

Genome size and base composition of five Pinus species from the Balkan region

  • F. Bogunic
  • E. Muratovic
  • S. C. Brown
  • S. Siljak-Yakovlev
Genetics and Genomics


The 2C DNA content and base composition of five Pinus (2n=24) species and two Pinus subspecies from the Balkan region have been estimated by flow cytometry. P. heldreichii (five populations) and P. peuce (one population) were assessed for the first time, as also were subspecies of P. nigra (three populations—two of subspecies nigra and one of subspecies dalmatica) along with P. sylvestris, and P. mugo from the same region. The 2C DNA values of these Pinus ranged from 42.5 pg to 54.9 pg (41.7–53.8×10bp), and the base composition was quite stable (about 39.5% GC). Significant differences were observed between two subspecies of P. nigra and even between two populations of subsp. nigra. The two other species (P. sylvestris and P. mugo) had 2C values of 42.5 pg and 42.8 pg, respectively, while that of P. peuce was 54.9 pg. These genome sizes are in accordance with published values except for P. sylvestris, which was 20% below estimates made by other authors.


Pinus Flow cytometry Balkan area Interspecific and intraspecific variations 



The authors kindly acknowledge Dalibor Ballian, Dr. Edita Solic, and J. Buselic for collecting material and Odile Robin, Olivier Cartrice, and Marie-Thérèse Crosnier for their technical support.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • F. Bogunic
    • 1
    • 2
  • E. Muratovic
    • 2
  • S. C. Brown
    • 3
  • S. Siljak-Yakovlev
    • 2
    • 4
  1. 1.Faculty of ForestryUniversity of SarajevoSarajevoBosnia and Herzegovina
  2. 2.Laboratory for research and protection of endemic resources, Department of Biology, Faculty of SciencesUniversity of SarajevoSarajevoBosnia and Herzegovina
  3. 3.Institut des Sciences du VégétalCNRS UPR 2355Gif-sur-YvetteFrance
  4. 4.Ecologie, Systématique, EvolutionUniversité Paris-SudOrsayFrance

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