Abstract
Cell suspension cultures of Saussurea medusa were grown in shake flasks and a 5-l stirred tank bioreactor. Biomass and jaceosidin distribution in cell aggregates of different sizes were investigated during the cultivation period. The results showed that on day 10, jaceosidin accumulation showed an increase with increasing size of the cell aggregate to 4 mm in diameter, with the highest jaceosidin accumulation being 12.2 mg/g. An inverse tendency was observed with cell aggregates larger than 4 mm in diameter, with the lowest accumulation being 3.1 mg/g. However, all of the cell aggregates, despite their size, synthesized almost the same amount of jaceosidin at day 12. Oxygen diffusion limitation and cell-cell contact may explain this behavior. In comparison with cells cultivated in shake flasks, decreased biomass and decreased jaceosidin concentration were observed when the cells were cultivated in a stirred tank bioreactor. The sublytic effects caused by the hydrodynamic stress in combination with insufficient nutrients in the bioreactor may cause cell damage.
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Abbreviations
- * :
-
Multiplication sign
- BA :
-
N6-Benzyladenine
- 2,4-D :
-
2,4-Dichlorophenoxyacetic acid
- HPLC :
-
High performance liquid chromatography
- IAA :
-
Indole-3- acetic acid
- NAA :
-
α-Naphthaleneacetic acid
- TLC :
-
Thin-layer chromatography
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Acknowledgements
We thank Prof. Rob Verpoorte, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands, for critical reading of the manuscript, and Prof. Jia Zhong-Jian, Department of Chemistry of Lanzhou University, Qing Hai province, Lanzhou City, P.R. China, for his generous gifts of standard chemicals (jaceosidin).
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Communicated by G.C. Phillips
This project was supported by the National Science Foundation of China (no. 39970896)
D. Zhao and Y. Huang have contributed equally to this article.
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Zhao, D., Huang, Y., Jin, Z. et al. Effect of aggregate size in cell cultures of Saussurea medusa on cell growth and jaceosidin production. Plant Cell Rep 21, 1129–1133 (2003). https://doi.org/10.1007/s00299-003-0631-8
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DOI: https://doi.org/10.1007/s00299-003-0631-8