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Auxin deprivation induces a developmental switch in maize somatic embryogenesis involving redistribution of microtubules and actin filaments from endoplasmic to cortical cytoskeletal arrays

  • Cell Biology and Morphogenesis
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Abstract

A developmental switch from non-polar pre-embryogenic units to polarized transition units in maize embryogenic callus is caused by auxin deprivation from the culture medium. This switch is accompanied by cytoskeletal rearrangements in embryogenic cells. An immunofluorescence study revealed prominent endoplasmic microtubules and actin filament meshworks radiating from the nuclear surfaces in pre-embryogenic cells growing on medium supplemented with auxin. On the other hand, parallel-organized cortical microtubules and cortical actin filament networks are inherently associated with polarized embryogenic cells of transition units growing on medium without auxin. These results indicate that fine-tuning of the dynamic equilibrium between endoplasmic and cortical cytoskeletal arrays is important for progress in somatic embryogenesis.

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Abbreviations

AGP :

Arabinogalactan protein

BSA :

Bovine serum albumin

2,4-D :

2, 4-Dichlorophenoxyacetic acid

FITC:

Fluorescein isothiocyanate

MS :

Murashige and Skoog medium

PBS :

Phosphate-buffered saline

SB :

Stabilizing buffer

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Acknowledgements

This work was supported by a research fellowship from the Alexander von Humboldt Foundation (Bonn, Germany) to J. Š., and by VEGA grant Nr. 2/6016/99 from the Slovak Ministry of Science and Education.

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Author notes

  1. J. Šamaj

    Present address: Institute of Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany

Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, 950 07 , Nitra, Slovakia

    J. Šamaj & A. Pretová

  2. Institute of Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany

    F. Baluška & D. Volkmann

Authors
  1. J. Šamaj
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  2. F. Baluška
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  3. A. Pretová
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  4. D. Volkmann
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Corresponding author

Correspondence to J. Šamaj.

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Communicated by H. Lörz

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Šamaj, J., Baluška, F., Pretová, A. et al. Auxin deprivation induces a developmental switch in maize somatic embryogenesis involving redistribution of microtubules and actin filaments from endoplasmic to cortical cytoskeletal arrays. Plant Cell Rep 21, 940–945 (2003). https://doi.org/10.1007/s00299-003-0611-z

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  • DOI: https://doi.org/10.1007/s00299-003-0611-z

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