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The S haplotypes lacking SLG in the genome of Brassica rapa

  • Genetics and Genomics
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Abstract

Self-incompatibility (SI) discriminating self- and non-self pollen is regulated by S-locus genes in Brassica. In most of the S haplotypes, a highly polymorphic S-locus glycoprotein (SLG) gene is tightly linked to genes for the SI determinants, S-receptor kinase (SRK) and SP11, although the precise function of SLG in SI has not been clarified. In the present study, we performed DNA gel blot analysis for S 32, S 33, and S 36 haplotypes of Brassica rapa showing normal SI phenotypes and concluded that there might be no SLG in their genome. RNA gel blot analysis of the SLG-less S haplotypes indicated the possible existence of eSRK transcripts in the stigma. These three S haplotypes are useful resources to discern the molecular mechanism of the SI reaction without SLG.

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Acknowledgements

We thank Akiko Ito, Takayuki Nakanowatari, Ayako Chiba, Eri Abe, Misako Shinkai, and Rina Kikuta of Iwate University for their help. This work was supported in a part by Grants-in-Aid for Special Research on Priority Areas B (nos. 11238201 and 14360002) from the Ministry of Education, Science, Culture and Sports, Japan, to M.W., and by a grant from the Intelligent Cosmos Academic Foundation to M.W. Y.T. is a recipient of Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.

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Authors and Affiliations

  1. Division of Natural Science, Osaka Kyoiku University, Kashiwara, 582-8582, Osaka , Japan

    G. Suzuki

  2. Laboratory of Plant Breeding, Faculty of Agriculture, Iwate University, 3-18-8, 020-8550, Morioka , Japan

    T. Kakizaki, Y. Takada & M. Watanabe

  3. Graduate School of Biological Sciences, Nara Institute of Science and Technology, 630-0101, Ikoma, Japan

    H. Shiba, S. Takayama & A. Isogai

Authors
  1. G. Suzuki
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  2. T. Kakizaki
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  3. Y. Takada
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  4. H. Shiba
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  5. S. Takayama
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  6. A. Isogai
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  7. M. Watanabe
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Corresponding author

Correspondence to M. Watanabe.

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Communicated by H. Uchimiya

G. Suzuki and T. Kakizaki contributed equally to this work.

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Suzuki, G., Kakizaki, T., Takada, Y. et al. The S haplotypes lacking SLG in the genome of Brassica rapa . Plant Cell Rep 21, 911–915 (2003). https://doi.org/10.1007/s00299-003-0598-5

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  • DOI: https://doi.org/10.1007/s00299-003-0598-5

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