Skip to main content
SpringerLink
Log in

Adventitious shoot regeneration from in vitro cultured leaves of London plane tree (Platanus acerifolia Willd.)

  • Cell Biology and Morphogenesis
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Adventitious shoots were successfully regenerated from leaf explants of in vitro cultures of Platanus acerifolia Willd. The leaves of three clones (genotypes), designated as PH1, PH2 and PC, respectively, were wounded by three to four transverse cuts through the midvein and cultured on 26 media based on Murashige and Skoog (MS) basal medium, containing different concentrations of 6-benzylaminopurine (BAP) in combination with different concentrations of indole-3-butyric acid (IBA). The highest regeneration rate (>90%) and the largest number of shoot clumps per regenerating leaf (>4 shoot clumps/explant) were obtained with leaves of genotype PH2 cultured on MS basal medium supplemented with 17.76 μM BAP and 4.92 μM IBA. The other two genotypes, PH1 and PC, showed very low capability of shoot regeneration (<10%) on all the media tested. Shoots on leaf explants originated mainly from callus that developed around the cut end of petioles and along the cuts across the midvein. The regenerated shoots were micropropagated, rooted and transplanted to the soil successfully.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–f.

Similar content being viewed by others

Abbreviations

BAP:

6-Benzylaminopurine

GA3 :

Gibberellic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog (1962)

NAA:

Naphthalene acetic acid

PGR:

Plant growth regulator

PPF:

Photosynthetic photon flux

References

  • Caboni E, Tonelli MG, Lauri P, Angeli SD, Damiano C (1999) In vitro shoot regeneration from leaves of wild pear. Plant Cell Tissue Organ Cult 59:1–7

    CAS  Google Scholar 

  • Estruch JJ, Carozzi NB, Desai N, Duck NB, Warren GW, Koziel MG (1997) Transgenic plants: an emerging approach to pest control. Nature/Biotechnology 15:137–141

    Google Scholar 

  • Gjuleva V, Atanasov A (1994) Micropropagation of Platanus acerifolia in vitro. Silv Genet 43(4):215–218

    Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant cell tissue culture. Plant Cell Tissue Organ Cult 33:105–121

    CAS  Google Scholar 

  • Jain SM, Minocha SC (1999) Molecular biology of woody plants. Kluwer, Dordrecht

  • Jouanin L, Brasileiro ACM, Leple JC, Pilate G, Cornu D (1993) Genetic transformation: a short review of methods and their applications, results and perspectives for forest trees. Ann Sci For 50:325–336

    Google Scholar 

  • Kim MK, Sommer HE, Bongarten BC, Merkle SA (1997) High-frequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. by thidiazuron. Plant Cell Rep 16:536–540

    Article  CAS  Google Scholar 

  • Liu G-F, Huang J, Chen L-Q, Bao M-Z (in press) Plant regeneration from excised hypocotyl explants of Platanus acerifolia Willd. In vitro Cell Dev Biol Plant 38:558–563

  • Lu CY (1993) The use of thidiazuron in tissue culture. In vitro Cell Dev Biol 29:92–96

    Google Scholar 

  • Matasci M, Gessler C (1997) A fungus menaces the London plane tree. Acta Vet Hung 45(2):69–75

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for growth and rapid bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • SAS Institute (1995) SAS/STAT user's guide, release 6.12. SAS Institute, Cary, N.C.

  • Shuerman PL, Dandekar AM (1993) Transformation of temperate woody crops: progress and potentials. Sci Hortic 55:101–124

    Google Scholar 

  • Strauss SH, Rottmann WH, Brunner AM, Sheppard LA (1995) Genetic engineering of reproductive sterility in forest trees. Mol Breed 1:5–26

    CAS  Google Scholar 

  • Subiza J, Cabrera M, Valdivieso R, Subiza JL, Jerez M, Jimenez JA, Narganes MJ, Subiza E (1994) Seasonal asthma caused by airborne Platanus pollen. Clin Exp Allergy 24(12):1123–1129

    CAS  PubMed  Google Scholar 

  • Varela S, Subiza J, Subiza JL, Rodriguez R, Garcia B, Jerez M, Jimenez JA, Panzani R (1997) Platanus pollen as an important cause of pollinosis. J Allergy Clin Immunol 100(1):748–754

    CAS  PubMed  Google Scholar 

  • Vigouroux A (1992) Preliminary results for obtaining a plane tree resistant to canker stain and adapted to European conditions. Acta Hortic 320:91–96

    Google Scholar 

  • Wei Z-M, Xu Z-H (1991) Mesophyll protoplast culture and plant regeneration of oriental planetree (Platanus orientalis). Acta Bot Sin 33(1):813–818

    CAS  Google Scholar 

  • Walter JM (1946) Canker stain of plane trees. Circular no. 742. United States Department of Agriculture, Washington, D.C.

  • Walter JM, Rex EG, Schreiber R (1952) The rate of progress and destructiveness of canker stain of plane trees. Phytopathology 42:236–239

    Google Scholar 

  • Zuker A, Ahroni A, Shejtman H, Vainstein A (1997) Adventitious shoot regeneration from leaf explants of Gypsophila paniculata L. Plant Cell Rep 16:775–778

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research is partially supported by the Bureau of Science and Technology, Wuhan. We thank all the colleagues in our laboratory for constructive discussion and technical support.

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Genetic Improvement, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China

    G. Liu & M. Bao

Authors
  1. G. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Bao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Bao.

Additional information

Communicated by: S.A. Merkle

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, G., Bao, M. Adventitious shoot regeneration from in vitro cultured leaves of London plane tree (Platanus acerifolia Willd.). Plant Cell Rep 21, 640–644 (2003). https://doi.org/10.1007/s00299-002-0569-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-002-0569-2

Keywords

Search

Navigation