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Production of haploids from anther culture of banana [Musa balbisiana (BB)]

Abstract

We report here, for the first time, the production of haploid plants of banana Musa balbisiana (BB). Callus was induced from anthers in which the majority of the microspores were at the uninucleate stage. The frequency of callus induction was 77%. Callus proliferation usually preceded embryo formation. About 8% of the anthers developed androgenic embryos. Of the 147 plantlets obtained, 41 were haploids (n=x=11). The frequency of haploid production depended on genotypes used: 18 haploid plants were produced from genotype Pisang klutuk, 12 from Pisang batu, seven from Pisang klutuk wulung and four from Tani. The frequency of regeneration was 1.1%, which was based on the total number of anthers cultured. Diploid plants (2n=2x=22) were also observed in the regenerated plants. The haploid banana plants that were developed will be important material for the improvement of banana through breeding programmes.

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Abbreviations

BAP :

Benzylaminopurine

CIRAD :

Centre de coopération internationale en recherche agronomique pour le développement

DAPI :

4,6-Diamidino-2-phenylindole

IAA :

Indole-3-acetic acid

MOPS :

3-(N- morpholino) propanesulfonic acid

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Acknowledgements

We thank Dr. M.V. Rajam, University of Delhi South Campus, New Delhi for reading the manuscript and Mr. D. Froger for his help with the photography. This investigation was generously supported by the European Union (INCO-DC-Contract no. IC18-CT97-02-04).

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Correspondence to A. Assani.

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Communicated by H. Lörz

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Assani, A., Bakry, F., Kerbellec, F. et al. Production of haploids from anther culture of banana [Musa balbisiana (BB)]. Plant Cell Rep 21, 511–516 (2003). https://doi.org/10.1007/s00299-002-0557-6

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  • DOI: https://doi.org/10.1007/s00299-002-0557-6

Keywords

  • Musa balbisiana
  • Anther culture
  • Androgenesis
  • Haploids
  • Plant regeneration