Skip to main content
SpringerLink
Log in

Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites

  • Physiology and Biochemistry
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract.

Hairy root cultures were established from stem and leaf explants of Azadirachta indica A. Juss (neem) following infection with Agrobacterium rhizogenes. Transformation was confirmed using polymerase chain reaction analysis. The cultures had a relatively fast growth rate (0.134 g dry weight day–1) and showed a 100-fold increase in biomass over a 4-week culture period. Both the roots and cultivation medium were antifeedant as determined by a no-choice bioassay using the desert locust, Schistocerca gregaria. Azadirachtin, nimbin, salannin, 3-acetyl-1-tigloylazadirachtinin and 3-tigloylazadirachtol were detected in the hairy roots by supercritical fluid chromatography. The cultivation medium contained 3-acetyl-1-tigloylazadirachtinin, 3-tigloylazadirachtol and desacetylsalannin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Agriculture and Forestry, University of Aberdeen, MacRobert Building, 581 King Street, Aberdeen, AB24 5UA, UK, , , , ,

    E. Allan,  J. Eeswara &  T. Stuchbury

  2. Department of Chemistry, Keele University, Staffordshire, ST5 5BG, UK, , , , ,

    A. Jarvis &  E. Morgan

  3. Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB24 2TZ, UK, , , , ,

    A. Mordue (Luntz)

Authors
  1. E. Allan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Eeswara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Jarvis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Mordue (Luntz)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Stuchbury
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Electronic Publication

Rights and permissions

Reprints and permissions

About this article

Cite this article

Allan, .E., Eeswara, .J., Jarvis, .A. et al. Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites. Plant Cell Rep 21, 374–379 (2002). https://doi.org/10.1007/s00299-002-0523-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-002-0523-3

Search

Navigation