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Activity of the Arabidopsis blue copper-binding protein gene promoter in transgenic tobacco plants upon wounding

  • Biotic and Abiotic Stress
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Abstract

Wound-inducible gene promoters can be valuable in regulating the expression of resistance genes for insect and/or pathogen attack. The transcriptional regulatory region (from –1294 to +247) of a wound-inducible Arabidopsis gene encoding a blue copper-binding (BCB) protein was fused to the β-glucuronidase (GUS) gene in a plant transformation vector. Stable integration of the single Arabidopsis BCB/GUS gene into the tobacco genome resulted in strongly induced GUS activity upon wounding in the homozygous T2 plants. GUS gene expression was limited to the plant cells surrounding the wound site, showing localized induction. However, expression of the chimeric gene was delayed in time and reduced in intensity when compared with the response of BCB gene expression in Arabidopsis, suggesting that foreign gene promoter activity is reduced in the heterologous host

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Yang, K., Im, Y., Chung, G. et al. Activity of the Arabidopsis blue copper-binding protein gene promoter in transgenic tobacco plants upon wounding. Plant Cell Rep 20, 987–991 (2002). https://doi.org/10.1007/s00299-002-0436-1

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  • DOI: https://doi.org/10.1007/s00299-002-0436-1

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