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Plant Cell Reports

, Volume 20, Issue 9, pp 797–801 | Cite as

Development of an efficient plant regeneration system for Russian wildrye (Psathyrostachys juncea)

  •  Z. Wang
  •  D. Lehmann
  •  J. Bell
  •  A. Hopkins
Cell Biology and Morphogenesis

Abstract.

Russian wildrye [Psathyrostachys juncea (Fisch.) Nevski] is a cool-season forage grass with a broad adaptation to semi-arid regions of North America. In order to explore the potential of biotechnology for genetic improvement of this important forage species, we developed an efficient tissue culture system. Embryogenic calli were induced from mature embryos with an induction frequency in the range of 2–7%. The selected highly embryogenic calli allowed the regeneration of dozens of plants from a single callus. Individual embryogenic calli were then used to establish single genotype-derived suspension cultures. Eighteen embryogenic cell suspension lines were established from three cultivars (Bozoisky-Select, Sawki and Tetracan). A relatively high green plant regeneration frequency, up to 70%, was achieved from plated cell clusters of the established suspension cultures. The regenerated plants were fertile after two winters of vernalization in the field. This efficient plant regeneration system provides a solid basis for generating transgenic plants.

Russian wildrye Psathyrostachys juncea Embryogenic callus Embryogenic cell suspensions Plant regeneration 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  Z. Wang
    • 1
  •  D. Lehmann
    • 1
  •  J. Bell
    • 1
  •  A. Hopkins
    • 1
  1. 1.Forage Biotechnology Group, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA

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