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Rheumatology International

, Volume 18, Issue 5–6, pp 183–191 | Cite as

Expression of the tissue inhibitor of metalloproteinases (TIMP) gene family in normal and osteoarthritic joints

  • S. Su
  • J. Grover
  • P. J. Roughley
  • J. A. DiBattista
  • J. Martel-Pelletier
  • J.-P. Pelletier
  • M. Zafarullah
ORIGINAL ARTICLE

Abstract

The pathophysiological and biological significance of tissue inhibitor of the metalloproteinases-3 (TIMP-3) gene compared to other TIMPs was investigated in osteoarthritic (OA) human and normal bovine joint tissues. Human OA synovial fibroblasts in culture constitutively expressed TIMP mRNAs. TIMP-3, TIMP-1 and gelatinase A mRNAs were elevated in most human OA synovia over controls, while TIMP-2 expression was similar. TIMP-3 and TIMP-1 mRNAs present in bovine cartilage were inducible by serum factors. Transforming growth factor beta (TGF-β1) induced TIMP-3 RNA and protein in human OA and normal bovine chondrocytes. TIMP mRNAs were low (TIMP-1) or undetectable in human fetal chondrocytes but were expressed at all other ages. Thus, the two main joint tissues, synovial membranes and cartilage, express TIMP genes. Due to their matrix protecting activities, the presence of multiple TIMPs may be beneficial for normal joints, while increased TIMP-3 and TIMP-1 expression in arthritic joints may be associated with pathological remodeling.

Key words Arthritis Synovium Cartilage Gene expression Tissue inhibitors of metalloproteinases 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • S. Su
    • 1
  • J. Grover
    • 2
  • P. J. Roughley
    • 2
  • J. A. DiBattista
    • 1
  • J. Martel-Pelletier
    • 1
  • J.-P. Pelletier
    • 1
  • M. Zafarullah
    • 1
  1. 1.Centre de recherche du CHUM, Campus Notre-Dame and Département de Médecine, Université de Montréal, 1560 Sherbrooke est Montréal, Québec, Canada, H2L 4M1CA
  2. 2.Genetics Unit, Shriners Hospital for Children, and the Department of Surgery, McGill University, Montreal, Quebec, Canada H3G 1A6CA

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