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Rheumatology International

, Volume 38, Issue 5, pp 831–836 | Cite as

CXCL13 levels in serum but not in saliva are elevated in Asian Indian patients with primary Sjögren’s syndrome

  • Santosh Kumar Mandal
  • Pulukool Sandhya
  • Jayakanthan Kabeerdoss
  • Janardana Ramya
  • Gowri Mahasampath
  • Debashish Danda
Biomarkers
  • 57 Downloads

Abstract

Human and animal model studies suggest CXCL13 is a potential biomarker in primary Sjögren’s syndrome (pSS). CXCL13 has not been studied in Indian patients with pSS. pSS cases classified by American European Consensus Group (AECG) or American college of Rheumatology(ACR) 2012 criteria, attending rheumatology clinic between July 2014 and July 2015 were included. Hospital staff and healthy, non-blood related family members of patients constituted the control group. pSS cases underwent clinical evaluation, laboratory investigations, ESSDAI and ESSPRI scoring. Unstimulated saliva was collected by the spitting method. Salivary and serum CXCL13 were quantified by indirect ELISA. CXCL13 positivity was determined using Receiver Operator Characteristic (ROC) curve. STATA13.1 (StataCorpLP,Texas,USA) software was used for statistical analysis. In this study, 45 pSS cases and 42 healthy controls were recruited. In pSS, median levels of serum CXCL13, but not salivary CXCL13 was significantly higher as compared to the corresponding levels in healthy controls (p < 0.001). Using cutoff of 43.03 pg/ml obtained by ROC, serum CXCL13 positivity was seen in 31/43(72.1%) cases and 10/34 (29.4%) controls, respectively. Serum CXCL13 levels among pSS patients on treatment, treatment naïve patients and healthy controls were statistically different. Serum CXCL13 positivity was associated with oral symptoms (p = 0.02), ocular signs (p = 0.03) and hyperglobulinemia (p = 0.01). There was no association of salivary CXCL13 level with any of the clinical variables. While serum CXCL13 was elevated in pSS, salivary CXCL13 was not. In conclusion, serum CXCL13 positivity was found to be associated with oral symptoms, ocular signs and hyperglobulinemia in pSS.

Keywords

Saliva Serum CXCL13 Sjogren’s syndrome India 

Notes

Acknowledgements

We acknowledge the funding received from Christian Medical College (CMC) fluid research grant for the study.

Compliance with ethical standards

Conflict of interest

The authors report that they have no conflicts of interests.

Ethical approval

This study was approved by the Institutional review board (IRB) & Ethics committee and was conducted in accordance with the Helsinki declaration of 1975, as revised in 2008.

Informed consent

Informed consent has been obtained from all participants.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Immunology and RheumatologyChristian Medical CollegeVelloreIndia
  2. 2.Department of RheumatologyRabindranath Tagore international institute of cardiac sciencesKolkataIndia
  3. 3.St. Stephens Hospital, Tis HazariNew DelhiIndia
  4. 4.St. John’s Medical CollegeBengaluruIndia
  5. 5.Department of BiostatisticsChristian Medical CollegeVelloreIndia

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