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Genetic variations in the CLNK gene and ZNF518B gene are associated with gout in case–control sample sets

  • Original Article - Genes and Disease
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Abstract

A genome-wide association study of gout in European populations identified 12 genetic variants strongly associated with risk of gout, but it is unknown whether these variants are also associated with gout risk in Chinese populations. A total of 145 patients with gout and 310 healthy control patients were recruited for a case–control association study. Twelve SNPs of CLNK and ZNF518B gene were genotyped, and association analysis was performed. Odds ratios (ORs) with 95 % confidence intervals (CIs) were used to assess the association. Overall, we found four risk alleles for gout in patients: the allele “G” of rs2041215 and rs1686947 in the CLNK gene by dominant model (OR 1.66; 95 % CI 1.04–2.63; p = 0.031) (OR 2.19; 95 % CI 1.38–3.46; p = 0.001) and additive model (OR 1.39; 95 % CI 1.00–1.93; p = 0.049) (OR 1.67; 95 % CI 1.19–2.32; p = 0.003), respectively, and the allele “A” of rs10938799 and rs10016022 in ZNF518B gene by recessive model (OR 4.66; 95 % CI 1.44–15.09; p = 0.008) (OR 4.54; 95 % CI 1.23–16.76; p = 0.020). Further haplotype analysis showed that the TCATTCTGA haplotype of CLNK was more frequent among patients with gout (adjusted OR 0.48; 95 % CI 0.24–0.95; p = 0.036). Additionally, polymorphisms of rs2041215, rs10938799, and rs17467273 were also correlated with clinical pathological parameters. This study provides evidence for gout susceptibility genes, CLNK and ZNF518B, in a Chinese population, which may have potential as diagnostic and prognostic marker for gout patients.

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Acknowledgments

This work is supported by the Social Science Foundation of Chinese Ministry of Education (No. 12YJA850011), the Key Program of Natural Science Foundation of Xizang (Tibet) Autonomous Region (201122, 2014), and Major Training Program of Tibet University for Nationalities (No. 13myZP06). We would also like to thank BioScience Writers (BSW) for the assistance in the preparation of this manuscript.

Conflict of interest

The authors have no conflicts of interest to report.

Ethical standard

The use of samples was approved by the Human Research Committee of the Affiliated Hospital of Tibet University for Nationalities for Approval of Research Involving Human Subjects.

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Authors and Affiliations

  1. Key Laboratory for Basic Life Science Research of Tibet Autonomous Region, School of Medicine, Tibet University for Nationalities, Xianyang, 712082, Shaanxi, China

    Tian-bo Jin, Xugang Shi, Mutu Jiri, Na He, Dongya Yuan & Longli Kang

  2. Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Tibet University for Nationalities, #6 East Wenhui Road, Xianyang, 712082, Shaanxi, China

    Tian-bo Jin, Xugang Shi, Mutu Jiri, Na He, Dongya Yuan & Longli Kang

  3. School of Life Sciences, Northwest University, Xi’an, 710069, Shaanxi, China

    Tian-bo Jin & Yongchao Ren

  4. National Engineering Research Center for Miniaturized Detection Systems, Xi’an, 710069, Shaanxi, China

    Tian-bo Jin, Yongchao Ren & Tian Feng

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  1. Tian-bo Jin
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  2. Yongchao Ren
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Correspondence to Longli Kang.

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Tian-bo Jin and Yongchao Ren are joint first author.

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Jin, Tb., Ren, Y., Shi, X. et al. Genetic variations in the CLNK gene and ZNF518B gene are associated with gout in case–control sample sets. Rheumatol Int 35, 1141–1147 (2015). https://doi.org/10.1007/s00296-015-3215-3

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  • DOI: https://doi.org/10.1007/s00296-015-3215-3

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