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Absence of the SLC22A12 gene mutation in Turkish population with primary gout disease

Rheumatology International volume 33, pages 2921–2925 (2013)Cite this article

Abstract

The aim of the study was to examine whether SLC22A12 gene mutations might be influenced in primary gout disease. We included 32 patients with diagnosis of primary gout disease and 100 healthy volunteers. DNA was purified from peripheral blood, and all exons of the SLC22A12 gene were sequenced. We did not find any mutations in the SLC22A12 gene in all of the patients, but found 5 polymorphisms in exons 1 (g.T258C, g.C246T), 2 (g.C1246T) and 8 (g.T8011C) and in intron 9 (g.C8577T). However, we have not found any significant differences in the frequency of the individual genotypes between patients with primary gout disease and control group. In addition, the polymorphisms were not associated with hyperuricemia in our patients with primary gout disease. There was no previously reported mutation/polymorphisms of SLC22A12 gene in Turkish population. Our study is the first one in Turkish population and suggests that there is no association between primary gout disease and SLC22A12 gene polymorphisms. Sequence changes in the promotor and intronic regions of SLC22A12 gene should be investigated further with larger case groups.

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Acknowledgments

This study was supported by The Scientific Research Projects Management Unit of Akdeniz University, Antalya, Turkey. The authors would like to thank to Dr. Hakan Gülkesen for helps in statistical analysis.

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Affiliations

  1. Department of Medical Biology and Genetics, Faculty of Medicine, Akdeniz University, Antalya, Turkey

    Sezin Yakut, Zafer Cetin, Ayse E. Manguoglu & Guven Luleci

  2. Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Akdeniz University, Antalya, Turkey

    Mehmet Arman

  3. Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey

    Halide Akbas

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  1. Sezin Yakut
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  2. Zafer Cetin
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  3. Mehmet Arman
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  4. Halide Akbas
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  5. Ayse E. Manguoglu
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  6. Guven Luleci
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Correspondence to Sezin Yakut.

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Yakut, S., Cetin, Z., Arman, M. et al. Absence of the SLC22A12 gene mutation in Turkish population with primary gout disease. Rheumatol Int 33, 2921–2925 (2013). https://doi.org/10.1007/s00296-012-2533-y

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  • DOI: https://doi.org/10.1007/s00296-012-2533-y

Keywords

  • SLC22A12
  • Hyperuricemia
  • Uric acid
  • Gout