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Effects of pulsed electromagnetic fields on the mRNA expression of CAII and RANK in ovariectomized rats

Rheumatology International volume 32, pages 1527–1532 (2012)Cite this article

Abstract

The present study was designed to determine the effects of pulsed electromagnetic fields (PEMFs) on the mRNA expression of the carbonic anhydrase II (CAII) and receptor activator of NF-κB (RANK) in ovariectomized rats. A total of 48 SD rats were randomly divided into four groups [Sham, OVX, PEMFs, and E2 (premarin)], 12 rats in each group. Rats in the Sham group received sham ovariectomy, while rats in OVX, PEMFs, and E2 groups received ovariectomy. Twelve weeks following the surgery, rats (whole body) in the PEMFs group were exposed to PEMFs for 30 days with 3.8 mT, 8 Hz, and 40 min per day; rats in the E2 group were administered premarin (0.0625 mg/kg/d; intragastric administration 1–2 ml/100 g). Rats in the Sham and OVX groups housed in the same conditions. At the end of intervention, the level of serum estradiol of rats was measured. The gene expression of CAII and RANK in the left ilium of rats was determined with real-time fluorescent-nested quantitative polymerase chain reaction. Compared with the Sham group, the level of serum estradiol in the ovariectomized group was significantly decreased (P < 0.05); compared with the OVX group, CAIImRNA expression was significantly decreased in the PEMFs group and E group (P < 0.05, 0.01, respectively). Compared with the E group, RANKmRNA expression was significantly higher in the PEMFs group (P < 0.05); although RANKmRNA expression decreased in PEMFs group, no statistically significant difference was found between PEMF group and OVX group (P = 0.82). These data suggest that PEMFs could regulate the expression of CAIImRNA in ovariectomized rats.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China, Grant No. 30672215, China.

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Affiliations

  1. Department of Rehabilitation, Zhong Shang Hospital, Xiamen University, Xiamen, 361004, China

    Jian Chen

  2. Department of Rehabilitation, Wuhan General Hospital of Guangzhou Military General Hospital, Wuhan, 430000, China

    Li-Qun Huang

  3. Department of Rehabilitation, West China Hospital, Sichuan University, Chengdu, 610041, China

    Jian Chen, Li-Qun Huang, Qing-Jie Xia & Cheng-Qi He

  4. Province Key Laboratory of Rehabilitation, Sichuan, Chengdu, 610041, China

    Cheng-Qi He

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  1. Jian Chen
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  2. Li-Qun Huang
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  3. Qing-Jie Xia
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Correspondence to Cheng-Qi He.

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Chen, J., Huang, LQ., Xia, QJ. et al. Effects of pulsed electromagnetic fields on the mRNA expression of CAII and RANK in ovariectomized rats. Rheumatol Int 32, 1527–1532 (2012). https://doi.org/10.1007/s00296-010-1740-7

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  • DOI: https://doi.org/10.1007/s00296-010-1740-7

Keywords

  • Pulse electromagnetic
  • Fields estrogen
  • RANKmRNA
  • CAIImRNA