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Lack of evidence for association of two functional SNPs of CHI3L1 gene (HC-gp39) with rheumatoid arthritis

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Abstract

CHI3L1 gene encodes for a glycoprotein (HC-gp39 or YKL40) secreted by synovial fibroblasts, macrophages, neutrophil granulocytes and chondrocytes. Its expression is under the control of NF-kB. It is regarded as an acute phase protein, and its levels are significantly elevated in rheumatic diseases. Furthermore, HC-gp39 has been shown to be recognized by autoreactive T cells in rheumatoid arthritis. In the present study, we have examined two functional variants of the promoter region of CHI3L1 gene (CHI3L1-1 (rs4950928) and CHI3L1-2 (rs10399931) that have been reported earlier to be associated with schizophrenia and sarcoidosis. We used TaqMan allelic discrimination assays to study the genotypes of Hungarian patients with rheumatoid arthritis (n = 182) and of healthy controls (n = 194). No significant association of the investigated SNPs with the disease was found. Here we report that CHI3L1 SNPs, shown to be involved in the predisposition of schizophrenia, are not associated with rheumatoid arthritis.

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Acknowledgments

This work has been supported by grants OTKA 73247, OTKA77537, MRTN-CT-2005-019561, NKTH (National Research and Technology) TECH 08-A1/2-2008-0120, OTKA PD-76348. György Nagy and Peter Antal are J. Bolyai Research Fellows.

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1089, Budapest, Hungary

    Sanjeev K. Srivastava, Ágnes F. Semsei, György Nagy, András Falus & Edit I. Buzás

  2. Department of Measurement and Information Systems, Budapest University of Technology and Economics, Budapest, Pf. 91, 1521, Budapest, Hungary

    Péter Antal & Gábor Hullám

  3. Department of Rheumatology, County Hospital Kecskemét, Nyíri út 38. Pf. 149, 6000, Budapest, Hungary

    János Gál

  4. Polyclinic of the Hospitaller Brothers of St. John of God in Budapest, 1022, Budapest, Zsigmond köz 7, Hungary

    György Nagy

  5. Research Group for Inflammation Biology and Immunogenomics, Hungarian Academy of Sciences, Nagyvárad tér 4, 1089, Budapest, Hungary

    András Falus

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  1. Sanjeev K. Srivastava
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  2. Péter Antal
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  8. Edit I. Buzás
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Correspondence to Edit I. Buzás.

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Srivastava, S.K., Antal, P., Gál, J. et al. Lack of evidence for association of two functional SNPs of CHI3L1 gene (HC-gp39) with rheumatoid arthritis. Rheumatol Int 31, 1003–1007 (2011). https://doi.org/10.1007/s00296-010-1396-3

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  • DOI: https://doi.org/10.1007/s00296-010-1396-3

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