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Rheumatology International

, Volume 31, Issue 5, pp 595–603 | Cite as

Evaluation of inflammatory change and bone erosion using a murine type II collagen-induced arthritis model

  • Samjin Choi
  • Yeon-Ah Lee
  • Seung-Jae Hong
  • Gi-Ja Lee
  • Sung Wook Kang
  • Ji-Hye Park
  • Jeong-Hoon Park
  • Hun-Kuk ParkEmail author
Original Article

Abstract

The exact mechanism of rheumatoid arthritis (RA) is unclear, but a combination of genetic, environmental and hormonal factors is thought to be involved. This study examined the progressive arthritic reaction of murine type II collagen-induced arthritis (CIA), a representative animal model of RA. Arthritic reactions, including inflammation and bone erosion were examined using an objective non-invasive method. Two scoring systems were used to evaluate changes in cutaneous inflammation and bone erosion during RA progression. The severity of inflammation was evaluated by visual scoring of erythema and edema, while the degree of bone erosion was quantified by macroradiographical erosion analysis of specific bones. A significant difference was observed in both visual (P = 0.0001, n = 7) and radiographic (P < 0.0001, n = 7) examinations for the RA group as compared to the control. The relationship between inflammatory change and erosive change in bone showed a significant positive correlation, r = 0.9550 (P < 0.0001, n = 7). The overall rate of asymmetry was 25.23% in both fore- and hindpaws. The results generated from these experiments show that murine CIA is a promising model for elucidating the mechanism of RA. In addition, the results of this study may be used for monitoring RA progression as well as screening therapy efficacy in the joint pathology.

Keywords

Rheumatoid arthritis (RA) Type II collagen-induced arthritis (CIA) animal model Visual and radiographic examinations Inflammation Bone erosion Symmetry and asymmetry 

Abbreviations

ANOVA

Analysis of variance

AP

Anterior–posterior

CFA

Complete Freund adjuvant

CIA

Collagen-induced arthritis

IFA

Incomplete Freund adjuvant

MCP

Metacarpophalangeal

MTP

Metatarsophalangeal

PA

Posterior–anterior

PIP

Proximal interphalangeal

r

Pearson correlation coefficient

RA

Rheumatoid arthritis

ROI

Region of interest

SD

Standard deviation

TNF

Tumor necrosis factor

Notes

Acknowledgments

This study was supported by a research fund from Seoul R&BD (Grant # CR070054). The authors would like to express deep gratitude for the support.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Samjin Choi
    • 1
    • 2
  • Yeon-Ah Lee
    • 3
  • Seung-Jae Hong
    • 3
  • Gi-Ja Lee
    • 1
    • 2
  • Sung Wook Kang
    • 1
    • 4
  • Ji-Hye Park
    • 1
    • 2
  • Jeong-Hoon Park
    • 1
    • 2
  • Hun-Kuk Park
    • 1
    • 2
    • 5
    Email author
  1. 1.Department of Biomedical Engineering, College of MedicineKyung Hee UniversitySeoulRepublic of Korea
  2. 2.Healthcare Industry Research InstituteKyung Hee UniversitySeoulRepublic of Korea
  3. 3.Division of Rheumatology, Department of Internal Medicine, College of MedicineKyung Hee University Medical CenterSeoulRepublic of Korea
  4. 4.Department of Pharmacology, College of MedicineKyung Hee UniversitySeoulRepublic of Korea
  5. 5.Program of Medical EngineeringKyung Hee UniversitySeoulRepublic of Korea

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