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Terminal monosaccharide screening of synovial immunoglobulins G and A for the early detection of rheumatoid arthritis

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Abstract

The expressions of some terminal glycotopes of synovial immunoglobulins G, A, and M were analysed in relation to rheumatoid arthritis (RA) progression defined according to early and advanced radiological changes in patients’ hands. The relative amounts of terminal monosaccharides were determined by lectin-immunoblotting of immunoglobulin preparations using appropriate lectins able to recognize α2,6-linked (Sambucus nigra agglutinin) and α2,3-linked (Maackia amurensis agglutinin) sialic acid, galactose (Ricinus communis agglutinin I), N-acetylglucosamine (Griffonia simplicifolia agglutinin II) as well as α1,6-linked (Aleuria aurantia lectin), α1,3-linked (Lotus tetragonolobus agglutinin), and α1,2-linked (Ulex europaeus agglutinin) fucose. The results indicate differences between early and advanced RA stages in the terminal sugar exposition of synovial IgG and IgA, but not IgM. The galactose-deficient glycotope with exposed N-acetylglucosamine of the synovial 33.1-kDa IgG fragment appeared exclusively in the early stage of RA. In contrast, this glycotope of intact synovial IgG and IgA was present in both groups, although with higher proportions in advanced RA. The proportions of the sialyl and fucosyl determinants of intact synovial A and G immunoglobulins were clearly lower in the early RA group than in the advanced. The analysis of terminal oligosaccharide exposition in IgG, IgG fragments, and IgA present in the synovial fluid of RA patients might be applicable as a stage-specific marker in the diagnosis and therapy of RA patients.

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Abbreviations

RA:

Rheumatoid arthritis

SNA:

Lectin from Sambucus nigra

MAA:

Lectin from Maackia amurensis

RCA I:

Lectin from Ricinus communis

GSL II:

Lectin from Griffonia simplicifolia

AAL:

Lectin from Aleuria aurantia

LTA:

Lectin from Lotus tetragonolobus

UEA:

Lectin from Ulex europaeus

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Authors and Affiliations

  1. Department of Chemistry and Immunochemistry, Wrocław Medical University, Bujwida 44a, 50-345, Wrocław, Poland

    Ewa Maria Kratz & Iwona Kątnik-Prastowska

  2. Department of Rheumatology and Internal Diseases of the University Clinical Hospital, Wrocław Medical University, Borowska 213, 50-556, Wrocław, Poland

    Krzysztof Borysewicz

Authors
  1. Ewa Maria Kratz
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  2. Krzysztof Borysewicz
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  3. Iwona Kątnik-Prastowska
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Corresponding author

Correspondence to Ewa Maria Kratz.

Additional information

Some preliminary experiments described in this work were presented during the 2nd European Conference on Chemistry and Life Sciences, 2007, Wrocław, Poland.

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Kratz, E.M., Borysewicz, K. & Kątnik-Prastowska, I. Terminal monosaccharide screening of synovial immunoglobulins G and A for the early detection of rheumatoid arthritis. Rheumatol Int 30, 1285–1292 (2010). https://doi.org/10.1007/s00296-009-1139-5

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  • DOI: https://doi.org/10.1007/s00296-009-1139-5

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