Skip to main content

Advertisement

Log in

High frequency of Smith autoantibodies in Omani patients with systemic lupus erythematosus

  • Original Article
  • Published:
Rheumatology International Aims and scope Submit manuscript

Abstract

This study was conducted to investigate the frequency and significance of some antinuclear autoantibodies in Omani patients with systemic lupus erythematosus (SLE). Anti-nuclear antibodies (ANA), anti-double stranded-DNA (anti-dsDNA), and anti-Smith (anti-Sm) autoantibodies were investigated in 60 Omani patients clinically diagnosed with SLE according to the American College of Rheumatology Criteria. The SLE group included 57 females and 3 males with an average age of 26 years. In addition, a group of 60 healthy Omanis (26 females and 34 males; average age 25 years) was used as a control. ANA patterns and autoantibody profile were assayed by indirect immunofluorescence assay using Hep-2 cells and liver/kidney/stomach tissue, respectively. Anti-dsDNA were examined by enzyme-linked immunosorbent assays; anti-Sm antibodies were measured by immunoblotting technique. Out of the 60 SLE patients, 59/60 (98.3%) were seropositive for ANA. Anti-dsDNA and anti-Sm each was detected in 50/60 (83.3%) of the Omani patients. The homogenous pattern of ANA was detected in 30/60 (50%) of patients, whereas the frequency of fine-speckled and coarse-speckled was 16/60 (26.7%) and 6/60 (10%), respectively. High titers (≥1:320) of ANA was detected in 56/60 (93.3%) of SLE patients. High titers of anti-Sm were detected in 22/60 (33.3%) of patients. High titers (>100 IU/ml) of anti-dsDNA were detected in 40/60 (66.7%) of patients. In the control group, ANA were detected in 8/60 (13.3%) but at low titers, whereas anti-dsDNA and anti-Sm were not detected in the healthy control group. This study shows that anti-Sm is as important as the anti-dsDNA for confirming the diagnosis of SLE and that anti-Sm occurs at a much higher frequency (83.3%) than that reported in other populations indicating the importance of this specific autoantibody for the diagnosis and possibly prognosis of Omani SLE patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Haddouk S, Ben Ayed M, Baklouti S, Hachicha J, Bahloul Z, Masmoudi H (2005) Autoantibodies in systemic lupus erythematosus: spectrum and clinical associations. Pathol Biol (Paris) 53:311–317. doi:10.1016/j.patbio.2004.10.004

    CAS  Google Scholar 

  2. Stinton LM, Fritzler MJ (2007) A clinical approach to autoantibody testing in systemic autoimmune rheumatic disorders. Autoimmun Rev 7:77–84. doi:10.1016/j.autrev.2007.08.003

    Article  CAS  PubMed  Google Scholar 

  3. Petri M (2007) Monitoring systemic lupus erythematosus in standard clinical care. Best Pract Res Clin Rheumatol 21:687–697. doi:10.1016/j.berh.2007.01.003

    Article  PubMed  Google Scholar 

  4. Santos MJ, Capela S, Figueira R et al (2007) Characterization of a Portuguese population with systemic lupus erytematosus. Acta Reumatol Port 32:153–161

    CAS  PubMed  Google Scholar 

  5. Hocberg MC (1997) Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 40:1725. doi:10.1002/art.1780400928

    Article  Google Scholar 

  6. Petri M (2005) Review of classification criteria for systemic lupus erythematosus. Rheum Dis Clin North Am 31:245–254. doi:10.1016/j.rdc.2005.01.009

    Article  PubMed  Google Scholar 

  7. Tan EM, Cohen AS, Fries JF et al (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25:1271–1277. doi:10.1002/art.1780251101

    Article  CAS  PubMed  Google Scholar 

  8. Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA (2000) Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens (special article). Arch Pathol Lab Med 124:71–81

    CAS  PubMed  Google Scholar 

  9. Tan EM (1989) Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 44:93–151. doi:10.1016/S0065-2776(08)60641-0

    Article  CAS  PubMed  Google Scholar 

  10. Lam GK, Petri M (2005) Assessment of systemic lupus erythematosus. Clin Exp Rheumatol 23(5 Suppl 39):S120–S132

    CAS  PubMed  Google Scholar 

  11. Alballa SR (1995) Systemic lupus erythematosus in Saudi patients. J Clin Rheumatol 14:342–346. doi:10.1007/BF02208351

    Article  CAS  Google Scholar 

  12. Maraina CH, Kamaliah MD, Ishak M (2004) The autoantibody profile and its association with clinical manifestations in Malay SLE patients. Asian Pac J Allergy Immunol 22:33–37

    CAS  PubMed  Google Scholar 

  13. Arcavi M, Orfus G (2006) Prevalence of antinuclear envelope antibodies and their isotypes in sera positive for antinuclear antibodies. Medicina (B Aires) 66:327–331

    CAS  Google Scholar 

  14. Goulvestre C (2006) Antinuclear antibodies. Presse Med 35:287–295. doi:10.1016/S0755-4982(06)74572-9

    Article  PubMed  Google Scholar 

  15. Alsaleh J, Jassim V, Elsayed M, Saleh N, Harb D (2008) Clinical and immunological manifestations in 151 SLE patients living in Dubai. Lupus 17:62–66. doi:10.1177/0961203307084297

    Article  CAS  PubMed  Google Scholar 

  16. Arbuckle MR, James JA, Kohlhase KF, Rubertone MV, Dennis GJ, Harley JB (2001) Development of anti-dsDNA autoantibodies prior to clinical diagnosis of systemic lupus erythematosus. Scand J Immunol 54(1–2):211–219. doi:10.1046/j.1365-3083.2001.00959.x

    Article  CAS  PubMed  Google Scholar 

  17. Swaak AJG, Aarden LA, Statius van Eps LW, Feltamp TEW (1979) Anti-dsDNA and complements profile as prognostic guides in systemic lupus erythematosus. Arthritis Rheum 22:226–235. doi:10.1002/art.1780220304

    Article  CAS  PubMed  Google Scholar 

  18. ter Borg EJ, Horst G, Hummel EJ, Limburg PC, Kallenberg CG (1990) Measurement of increases in anti-double-stranded DNA antibody levels as a predictor of disease exacerbation in systemic lupus erythematosus: a long-term, prospective study. Arthritis Rheum 33(5):634–643. doi:10.1002/art.1780330505

    Article  CAS  PubMed  Google Scholar 

  19. Smeenk R, Brinkman K, van den Brink H, Termaat RM, Berden J, Nossent H et al (1990) Antibodies to DNA in patients with systemic lupus erythematosus: their role in the diagnosis, follow-up and the pathogenesis of the disease. Clin Rheumatol 9(1 Suppl 1):100–110. doi:10.1007/BF02205557

    Article  CAS  PubMed  Google Scholar 

  20. Migliorini P, Baldini C, Rocchi V, Bombardieri S (2005) Anti-Sm and anti-RNP antibodies. Autoimmunity 38:47–54. doi:10.1080/08916930400022715

    Article  CAS  PubMed  Google Scholar 

  21. Lu JJ, Chen DY, Hsieh CW, Lan JL, Lin FJ, Lin SH (2007) Association of Epstein–Barr virus infection with systemic lupus erythematosus in Taiwan. Lupus 16:168–175

    CAS  PubMed  Google Scholar 

  22. Alsuwaida A, Abdulkareem A, Alwakeel J (2007) The Gulf Survey on Anemia Management (GSAM 2005). Saudi J Kidney Dis Transpl 18:206–214

    PubMed  Google Scholar 

  23. Al-Jabri AA, Al Belushi MS, Nsanze H (2003) Frequency and levels of auto-antibodies in the adult healthy Omani individuals. Ann Saudi Med 23:372–375

    PubMed  Google Scholar 

  24. Al-Jabri AA, Richens ER (2001) Occurrence of auoantibodies within healthy Omani individual. Sultan Qaboos Univ J Med Sci 1:13–19

    Google Scholar 

  25. Al-Jabri AA, Al Belushi MS (2004) Anticardiolipin and antinuclear antibodies in the adult healthy Omani individuals. Saudi Med J 25:447–451

    Google Scholar 

  26. Johnson AE, Gordon C, Palmer RG, Bacon PA (1995) The prevalence and incidence of systemic lupus erythematosus in Birmingham, England: relationship to ethnicity and country of birth. Arthritis Rheum 38:551–558. doi:10.1002/art.1780380415

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We would like to express our thanks to Dr. Michele Monteil, Associate Professor and a Visiting Consultant of Immunology at SQU, for careful reading of the manuscript and making constructive suggestions.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ali A. Al-Jabri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Al-Jabri, A.A., Al-Gahdani, A.K. & Al-Shuaili, I. High frequency of Smith autoantibodies in Omani patients with systemic lupus erythematosus. Rheumatol Int 30, 51–56 (2009). https://doi.org/10.1007/s00296-009-0909-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00296-009-0909-4

Keywords

Navigation