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Differences between the early and advanced stages of rheumatoid arthritis in the expression of EDA-containing fibronectin

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Abstract

In the present study the expression of alternatively spliced synovial EDA-containing FN (EDA-FN) was analyzed in plasma and synovial fluid samples of rheumatoid arthritis (RA) patients with from 2 months to 20 years disease duration. The patient samples were divided into groups: those with early, established, and late progressive radiographic changes as well as those with different disease activity established by a CRP concentration. The expression of EDA-FN was determined by sandwich-type ELISA using a specific anti-EDA monoclonal antibody. The relative amount of EDA-FN in synovial fluid, but not in plasma samples, was significantly lower in the early RA group than in established and late RA. In contrast, its level did not correlate with the CRP concentration. Synovial EDA-FN might be used as a supplemented marker of early RA.

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Fig. 1

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Abbreviations

FN:

Fibronectin

EDA-FN:

Fibronectin with spliced EDA region

RA:

Rheumatoid arthritis

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Authors and Affiliations

  1. Department of Chemistry and Immunochemistry, Wroclaw Medical University, Bujwida 44a, 50-345, Wroclaw, Poland

    Magdalena Przybysz & Iwona Kątnik-Prastowska

  2. Department of Rheumatology and Internal Diseases of the University Clinical Hospital, Wroclaw Medical University, Borowska 213, 50-556, Wroclaw, Poland

    Krzysztof Borysewicz

Authors
  1. Magdalena Przybysz
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  2. Krzysztof Borysewicz
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  3. Iwona Kątnik-Prastowska
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Correspondence to Magdalena Przybysz.

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Przybysz, M., Borysewicz, K. & Kątnik-Prastowska, I. Differences between the early and advanced stages of rheumatoid arthritis in the expression of EDA-containing fibronectin. Rheumatol Int 29, 1397–1401 (2009). https://doi.org/10.1007/s00296-009-0866-y

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  • DOI: https://doi.org/10.1007/s00296-009-0866-y

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