Abstract
Osteoarthritis (OA) is largely considered to be a non-inflammatory disease, although there is compelling evidence that subclinical inflammation is a common event, even in the absence of acute inflammatory flares. In this study we analyze, by means of CD5 and CD69 expression, the infiltration and early activation of CD5+cells, mostly lymphocytes, in both synovial membrane and synovial fluid from advanced OA patients and compare them with samples from patients with rheumatoid arthritis and healthy controls. The number of infiltrating CD5+ cells in both synovial membrane and synovial fluid from patients with advanced OA was significantly reduced as compared with rheumatoid arthritis patients. However, synovial membrane and synovial fluid CD5+ cells on OA exhibited a phenotype with evidence of recent activation comparable to that observed in RA.
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Acknowledgments
The authors thank Orthopedic surgeons from the Hospital Clínico San Carlos for providing synovial membrane and synovial fluid samples. We wish to thank Dr. G. Roncador (CNIO, Madrid) for providing the antibody CD5/36 D2. We also thank E. Martín-Nieto (Department of Graphic Design) for her help in compiling the photographs. This work was supported by the grants FMMA and FIS 04/1698. Raquel Rollin holds a research contract of the “Fundación para la Investigación Biomédica-Hospital Clínico San Carlos Madrid”.
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Rollín, R., Marco, F., Jover, J.A. et al. Early lymphocyte activation in the synovial microenvironment in patients with osteoarthritis: comparison with rheumatoid arthritis patients and healthy controls. Rheumatol Int 28, 757–764 (2008). https://doi.org/10.1007/s00296-008-0518-7
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DOI: https://doi.org/10.1007/s00296-008-0518-7