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Defensins- and cathepsin G-ANCA in systemic lupus erythematosus

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Abstract

In this study, we examined the content of antineutrophil cytoplasmic antibodies (ANCA) against defensins and cathepsin G in sera from systemic lupus erythematosus (SLE) patients and their significance in estimating the activity of SLE. Defensins- and cathepsin G-ANCA in sera from 28 patients with SLE, eight patients with rheumatoid arthritis (RA) and eight patients with microscopic polyangitis (mPA) were measured by ELISA. Significantly increased defensins- and cathepsin G-ANCA were found in sera of patients with SLE and mPA when compared with the value of normal controls. Though significantly higher defensins- and cathepsin G-ANCA were detected in both active and inactive SLE patients, the value in active SLE patients was significantly higher than inactive SLE patients. After the therapy with high dose of prednisolone, the serum level of defensins- and cathepsin G-ANCA was decreased, and this decrease was sustained for at least 16 weeks. This study suggests that defensins- and cathepsin G-ANCA may serve as useful markers of the disease activity of SLE.

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References

  1. Gross WL, Csernok E (1995) Immunodiagnostic and pathophysiologic aspects of antineutrophil cytoplasmic antibodies in vasculitis. Curr Opin Rheumatol 7:11–19

    PubMed  CAS  Google Scholar 

  2. van der Woude FJ, Rasmussen N, Lobatto S, Wiik A, Permin H, van Es LA, van der Giessen M, van der Hem GK (1985) Autoantibodies against neutrophils and monocytes: tool for diagnosis and marker of disease activity in Wegener’s granulomatosis. Lancet 23:425–429

    Google Scholar 

  3. Jennette JC, Falk RJ, Andrassy K, Bacon PA, Churg J, Gross WL, Hagen EC, Hoffman GS, Hunder GG, Kallenberg CG (1994) Nomenclature of systemic vasculitides. Proposal of an international consensus conference. Arthritis Rheum 37:187–192

    PubMed  CAS  Google Scholar 

  4. Hoffman GS, Specks U (1998) Antineutrophil cytoplasmic antibodies. Arthritis Rheum 41:1521–1537

    PubMed  CAS  Google Scholar 

  5. Kuwana T, Sato Y, Saka M, Kondo Y, Miyata M, Obara K, Nishimaki T, Kasukawa R (2000) Anti-cathepsin G antibodies in the sera of patients with ulcerative colitis. J Gastroenterol 35:682–689

    Article  PubMed  CAS  Google Scholar 

  6. Halbwachs-Mecarelli L, Nusbaum P, Noel LH, Reumaux D, Erlinger S, Grunfeld JP, Lesavre P (1992) Antineutrophil cytoplasmic antibodies (ANCA) directed against cathepsin G in ulcerative colitis, Crohn’s disease and primary sclerosing cholangitis. Clin Exp Immunol 90:79–84

    Article  PubMed  CAS  Google Scholar 

  7. Sobajima J, Ozaki S, Okazaki T, Osakada F, Sumita S, Mori K, Nakao K (1996) Anti-neutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis: anti-cathepsin G and a novel antibody correlate with a refractory type. Clin Exp Immunol 105:120–124

    Article  PubMed  CAS  Google Scholar 

  8. Schnabel A, Csernok E, Isenberg DA, Mrowka C, Gross WL (1995) Antineutrophil cytoplasmic antibodies in systemic lupus erythematosus. Prevalence, specificities, and clinical significance. Arthritis Rheum 38:633–637

    PubMed  CAS  Google Scholar 

  9. Molnar K, Kovacs L, Kiss M, Husz S, Dobozy AG (2002) Pokorny Antineutrophil cytoplasmic antibodies in patients with systemic lupus erythematosus. Clin Exp Dermatol 27:59–61

    Article  PubMed  CAS  Google Scholar 

  10. Nassberger L, Sjoholm AG, Jonsson H, Sturfelt G, Akesson A (1990) Autoantibodies against neutrophil cytoplasm components in systemic lupus erythematosus and in hydralazine-induced lupus. Clin Exp Immunol 81:380–383

    Article  PubMed  CAS  Google Scholar 

  11. Tani K, Murphy WJ, Chertov O, Salcedo R, Koh CY, Utsunomiya I, Funakoshi S, Asai O, Herrmann SH, Wang JM, Kwak LW, Oppenheim JJ (2000) Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens. Int Immunol 12:691–700

    Article  PubMed  CAS  Google Scholar 

  12. Spitznagel JK (1990) Antibiotic proteins of human neutrophils. J Clin Invest 86:1381–1386

    Article  PubMed  CAS  Google Scholar 

  13. Tan EM, Cohen AS, Fries JF, Masai AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25:1271–1277

    PubMed  CAS  Google Scholar 

  14. Bombardier C, Gladman DD, Urowitz MB, Caron D, Chang CH (1992) Committee on prognosis studies in SLE. Derivation of the SLEDAI. Arthritis Rheum 35:630–640

    PubMed  CAS  Google Scholar 

  15. Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS (1988) The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 131:315–324

    Google Scholar 

  16. Jennette JC, Falk RJ, Andrassy K, Bacon PA, Churg J, Gross WL, Hagen EC, Hoffman GS, Hunder GG, Kallenberg CG (1994) Nomenclature of systemic vasculitides. Proposal of an international consensus conference. Arthritis Rheum 37:187–192

    PubMed  CAS  Google Scholar 

  17. Spronk PE, Bootsma H, Horst G, Huitema MG, Limburg PC, Tervaert JW, Kallenberg CG (1996) Antineutrophil cytoplasmic antibodies in systemic lupus erythematosus. Br J Rheumatol 35:625–631

    PubMed  CAS  Google Scholar 

  18. Nishiya K, Chikazawa H, Nishimura S, Hisakawa N, Hashimoto K (1997) Anti-neutrophil cytoplasmic antibody in patients with systemic lupus erythematosus is unrelated to clinical features. Clin Rheumatol 16:70–75

    Article  PubMed  CAS  Google Scholar 

  19. Folwaczny C, Jochum M, Noehl N, Schnettler D, Loeschke K, Frick H (1998) p-ANCA target antigens in ulcerative colitis. Z Gastroenterol 36:625–633

    PubMed  CAS  Google Scholar 

  20. Tervaert JW, Goldschmeding R, Elema JD, Limburg PC, van der Giessen M, Huitema MG, Koolen MI, Hene RJ, The TH, van der Hem GK (1990) Association of autoantibodies to myeloperoxidase with different forms of vasculitis. Arthritis Rheum 33:1264–1272

    Article  PubMed  CAS  Google Scholar 

  21. Chertov O, Michiel DF, Xu L, Wang JM, Tani K, Murphy WJ, Longo DL, Taub DD, Oppenheim JJ (1996) Identification of defensin-1, defensin-2, and CAP/azurocidin as T-cell chemoattractant proteins released from interleukin-8-stimulated neutrophils. J Biol Chem 271:2935–2940

    Article  PubMed  CAS  Google Scholar 

  22. Chertov O, Ueda H., Xu LL, Tani K, Murphy WJ, Wang JM, Howard OMZ, Sayers TJ, Oppenheim JJ (1997) Identification of human neutrophil-derived cathepsin G and azurocidin/CAP37 as chemoattractants for mononuclear cells and neutrophils. J Exp Med 186:739–747

    Article  PubMed  CAS  Google Scholar 

  23. Tani K, Murphy WJ, Chertov O, Salcedo R, Koh CY, Utsunomiya I, Funakoshi S, Asai O, Herrmann SH, Wang JM, Kwak LW, Oppenheim JJ (2000) Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens. Int Immunol 12:691–700

    Article  PubMed  CAS  Google Scholar 

  24. Tani K, Murphy WJ, Chertov O, Oppenheim JJ, Wang JM (2001) The neutrophil granule protein cathepsin G activates murine T lymphocytes and upregulates antigen-specific IG production in mice. Biochem Biophys Res Commun 282:971–976

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Ms. Tomoko Oka for assistance with lymphocyte preparation. This work was supported in part by a Grant-in-Aid for General Scientific Research (C) from the Ministry of Education, Science and Culture and the Ministry of Health and Welfare of Japan.

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Authors and Affiliations

  1. Department of Internal Medicine and Molecular Therapeutics, Institute of Health Biosciences, The University of Tokushima Graduate School, 18-15 Kuramoto-cho3, Tokushima, 770-8503, Japan

    Hiroyuki Tamiya, Kenji Tani, Jyunya Miyata, Keiko Sato, Tomoyuki Urata, Battur Lkhagvaa, Shinsaku Otsuka, Shizuka Shigekiyo & Saburo Sone

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  1. Hiroyuki Tamiya
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  2. Kenji Tani
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  3. Jyunya Miyata
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  4. Keiko Sato
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  6. Battur Lkhagvaa
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  7. Shinsaku Otsuka
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  9. Saburo Sone
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Correspondence to Saburo Sone.

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Tamiya, H., Tani, K., Miyata, J. et al. Defensins- and cathepsin G-ANCA in systemic lupus erythematosus. Rheumatol Int 27, 147–152 (2006). https://doi.org/10.1007/s00296-006-0173-9

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  • DOI: https://doi.org/10.1007/s00296-006-0173-9

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