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Inhibitory effect of cyclo-oxygenase-2 inhibitor on the production of matrix metalloproteinases in rheumatoid fibroblast-like synoviocytes

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Abstract

Cyclo-oxygenase (COX)-2 has been associated with inflammation in rheumatoid arthritis (RA), but its role in joint destruction remains unclear. In this study, we investigated the effect on cultured rheumatoid fibroblast-like synoviocytes (FLS) of the selective COX-2 inhibitor celecoxib on the expression of matrix metalloproteinases (MMPs), which play an important role in tissue degradation and angiogenesis in rheumatoid synovium. Treatment with nontoxic doses of celecoxib resulted in dose-dependent inhibition of MMP-1, -2, and -3 secretion from FLS when measured by enzyme-linked immunosorbent assay. Celecoxib suppressed proinflammatory cytokines (tumor necrosis factor-α and interleukin-1β) induced augmentation of the gelatinolytic activity on zymography. These results suggest that COX-2 inhibitors might influence matrix degradation or angiogenesis in RA by downregulating the expression of various MMPs in rheumatoid FLS.

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Acknowledgement

This study was supported by the Korea Science and Engineering Foundation, 2000 (no. 2000-000-00093-0).

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Authors and Affiliations

  1. Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-Dong, Kangnam-Ku, Seoul 135-710, Korea

    H. S. Cha, C. H. Jeon & E. M. Koh

  2. Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Korea

    K. S. Ahn & J. Kim

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  1. H. S. Cha
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  2. K. S. Ahn
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  3. C. H. Jeon
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  4. J. Kim
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  5. E. M. Koh
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Correspondence to E. M. Koh.

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Cha, H.S., Ahn, K.S., Jeon, C.H. et al. Inhibitory effect of cyclo-oxygenase-2 inhibitor on the production of matrix metalloproteinases in rheumatoid fibroblast-like synoviocytes. Rheumatol Int 24, 207–211 (2004). https://doi.org/10.1007/s00296-003-0359-3

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  • DOI: https://doi.org/10.1007/s00296-003-0359-3

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