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PCR cloning and detection of point mutations in the eburicol 14a-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a “recalcitrant” fungus

Abstract

Molecular studies of some micro-organisms are hampered by the difficulty of obtaining sufficient amounts of nucleic acids. A cloning strategy based on PCR has therefore been used to clone the eburicol 14α-demethylase (CYP51) gene of the obligate fungus Erysiphe graminis f. sp. hordei (Egh) using minute amounts of genomic DNA. The CYP51 gene encodes the enzymatic target of a major group of fungicides. Sequencing CYP51 from different Egh isolates revealed the occurrence of two alleles for this gene. An allele-specific PCR assay was developed to detect each CYP51 allele.

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Received: 30 July / 3 October 1998

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Délye, C., Bousset, L. & Corio-Costet, MF. PCR cloning and detection of point mutations in the eburicol 14a-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a “recalcitrant” fungus. Curr Genet 34, 399–403 (1998). https://doi.org/10.1007/s002940050413

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  • DOI: https://doi.org/10.1007/s002940050413

  • Key wordsErysiphe graminis
  • Polymerase chain reaction
  • Cytochrome P450
  • Fungicide