Abstract
Northern-blot analysis of RNA isolated from Sclerotinia sclerotiorum grown on either glucose or polygalacturonate as the sole carbon source showed that pg1, encoding a neutral polygalacturonase, was not expressed during growth in both media. In contrast, transcripts of this gene were detected during infection of sunflower germlings. Analysis of the promoter sequence revealed a number of cis-acting sequences known to regulate the expression of many fungal promoters. Protein-DNA-binding experiments showed that proteins extracted from mycelia grown on polygalacturonate or glucose interacted with different regions of the promoter. The GST-CREA fusion protein, containing the two zinc fingers of the Aspergillus nidulans repressor CREA involved in carbon catabolite repression, forms several complexes with DNA fragments carrying the consensus 5′-SYGGRG-3′. These results suggest that a CREA homolog may be involved in the regulation of pg1.
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Received: 21 February/19 April 1996
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Reymond-Cotton, P., Fraissinet-Tachet, L. & Fèvre, M. Expression of the Sclerotinia sclerotiorum polygalacturonase pg1 gene: possible involvement of CREA in glucose catabolite repression. Curr Genet 30, 240–245 (1996). https://doi.org/10.1007/s002940050127
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DOI: https://doi.org/10.1007/s002940050127