Advances in linking polyketides and non-ribosomal peptides to their biosynthetic gene clusters in Fusarium

Nielsen, Mikkel Rank; Sondergaard, Teis Esben; Giese, Henriette; Sørensen, Jens Laurids

doi:10.1007/s00294-019-00998-4

Review
Published: 28 May 2019

Advances in linking polyketides and non-ribosomal peptides to their biosynthetic gene clusters in Fusarium

Current Genetics volume 65, pages 1263–1280 (2019)Cite this article

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Abstract

The eukaryotic ascomycete genus Fusarium comprises many species capable of producing secondary metabolites important for agriculture, health, and biotechnology. Filamentous fungi share common physiological features, but even within Fusarium, there are significant differences that affect the success of biotechnological methods used to unravel biosynthetic pathways. The aim of this review is to describe the different methods that have successfully been used throughout the genus Fusarium to identify the products of novel biosynthetic pathways. The results are presented in tables to give the reader an overview and thereby enable the selection of the most appropriate method to the problem, regarding both species and target products. Significant work has gone into characterization of the underlying molecular genetics of secondary metabolites, but still, the products of only 25–30% of predicted gene clusters have been identified. In this review, we highlight existing knowledge and encourage the development of new techniques and strategies to provide access to the many unknown polyketide and non-ribosomal peptide products that await discovery in Fusarium.

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Acknowledgements

This study was funded by Novo Nordisk Foundation (Grant number NNF15OC0016028) to MRN.

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Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700, Esbjerg, Denmark
Mikkel Rank Nielsen & Jens Laurids Sørensen
Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Plads 7H, 9220, Aalborg Ø, Denmark
Teis Esben Sondergaard & Henriette Giese

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Mikkel Rank Nielsen
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Henriette Giese
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Nielsen, M.R., Sondergaard, T.E., Giese, H. et al. Advances in linking polyketides and non-ribosomal peptides to their biosynthetic gene clusters in Fusarium. Curr Genet 65, 1263–1280 (2019). https://doi.org/10.1007/s00294-019-00998-4

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Received: 23 January 2019
Revised: 20 May 2019
Accepted: 22 May 2019
Published: 28 May 2019
Issue Date: December 2019
DOI: https://doi.org/10.1007/s00294-019-00998-4

Keywords

Fusarium
Secondary metabolites
Gene cluster
PKS
NRPS
Natural products