CreA-independent carbon catabolite repression of cellulase genes by trimeric G-protein and protein kinase A in Aspergillus nidulans

Abstract

Cellulase production in filamentous fungi is repressed by various carbon sources. In our preliminary survey in Aspergillus nidulans, degree of de-repression differed depending on carbon sources in a mutant of creA, encoding the transcriptional repressor for carbon catabolite repression (CCR). To further understand mechanisms of CCR of cellulase production, we compared the effects of creA deletion with deletion of protein kinase A (pkaA) and G (ganB) genes, which constitute a nutrient sensing and signaling pathway. In plate culture with carboxymethyl cellulose and d-glucose, deletion of pkaA and ganB, but not creA, led to significant de-repression of cellulase production. In submerged culture with cellobiose and d-glucose or 2-deoxyglucose, both creA or pkaA single deletion led to partial de-repression of cellulase genes with the highest level by their double deletion, while ganB deletion caused de-repression comparable to that of the creA/pkaA double deletion. With ball-milled cellulose and d-glucose, partial de-repression was detected by deletion of creA but not of pkaA or ganB. The creA/pkaA or creA/ganB double deletion led to earlier expression than the creA deletion. Furthermore, the effect of each deletion with d-xylose or L-arabinose as the repressing carbon source was significantly different from that with d-glucose, d-fructose, and d-mannose. Consequently, this study revealed that PkaA and GanB participate in CreA-independent CCR and that contribution of CreA, PkaA, and GanB in CCR differs depending on the inducers, repressing carbon sources, and culture conditions (plate or submerged). Further study of CreA-independent mechanisms is needed to fully understand CCR in filamentous fungi.

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Acknowledgements

This work was partially supported by the Program for Promotion of Basic and Applied Research for Innovations in Bio-oriented Industry and by the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry. It was also supported by JSPS KAKENHI (Grant Numbers JP 18H02125 and JP 17H06763) and funding from Institute for Fermentation, Osaka (IFO) and Noda Institute for Scientific Research.

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Correspondence to Tetsuo Kobayashi.

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Kunitake, E., Li, Y., Uchida, R. et al. CreA-independent carbon catabolite repression of cellulase genes by trimeric G-protein and protein kinase A in Aspergillus nidulans. Curr Genet 65, 941–952 (2019). https://doi.org/10.1007/s00294-019-00944-4

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Keywords

  • Aspergillus nidulans
  • Carbon catabolite repression
  • Cellulase
  • PkaA
  • GanB