Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
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In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited. Nonetheless, TEs have been initially proposed as positive control elements acting in synergy with the host. Nowadays, it is well known that TE movement into host genome comprises an important evolutionary mechanism capable of increasing the adaptive fitness. As insights into TE functioning are increasing day to day, the manipulation of transposition has raised an interesting possibility of setting the host functions, although the lack of appropriate genome engineering tools has unpaved it. Fortunately, the emergence of genome editing technologies based on programmable nucleases, and especially the arrival of a multipurpose RNA-guided Cas9 endonuclease system, has made it possible to reconsider this challenge. For such purpose, a particular type of transposons referred to as miniature inverted-repeat transposable elements (MITEs) has shown a series of interesting characteristics for designing functional drivers. Here, recent insights into MITE elements and versatile RNA-guided CRISPR/Cas9 genome engineering system are given to understand how to deploy the potential of TEs for control of the host transcriptional activity.
KeywordsCRISPR/Cas9 TE-based drives Genome editing Miniature inverted transposable elements Transcriptional control Transposable element amplification
The author would like to thank Marcela Rivarola and Cristian Arellano for grammatical corrections. Also, I wish to thank Jimena Ingaramo who provided support in the preparation of the figures.
Compliance with ethical standards
Conflict of interest
The author declares that has no conflict of interest.
- Castelletti S, Tuberosa R, Pindo M, Salvi S (2014) A MITE transposon insertion is associated with differential methylation at the maize flowering time QTL Vgt1. G3 4:805–812Google Scholar
- Chen J, Hu Q, Lu C, Kuang H (2014) Evolutionary genomics of miniature inverted-repeat transposable elements (MITEs) in plants. In: Pontarotti P (ed) Evolutionary biology: genome evolution, speciation, coevolution and origin of life. Springer, Cham, pp 157–168Google Scholar
- Feschotte C, Zhang X, Wessler S (2002b) Miniature inverted-repeat transposable elements (MITEs) and their relationship with established DNA transposons. In: Craig NL, Craigie R, Gellert M, Lambowitz AM (eds) Mobile DNA II, vol 35. American Society for Microbiology Press, Washington, DC, pp 1147–1158CrossRefGoogle Scholar
- Sakuma T, Yamamoto T (2015) CRISPR/Cas9: the leading edge of genome editing technology. Targeted genome editing using site-specific nucleases. Springer, Tokyo, pp 25–41Google Scholar