Current Genetics

, Volume 62, Issue 4, pp 887–896

Sequencing and analysis of the complete organellar genomes of Parmales, a closely related group to Bacillariophyta (diatoms)

  • Naoyuki Tajima
  • Kenji Saitoh
  • Shusei Sato
  • Fumito Maruyama
  • Mutsuo Ichinomiya
  • Shinya Yoshikawa
  • Ken Kurokawa
  • Hiroyuki Ohta
  • Satoshi Tabata
  • Akira Kuwata
  • Naoki Sato
Original Article

DOI: 10.1007/s00294-016-0598-y

Cite this article as:
Tajima, N., Saitoh, K., Sato, S. et al. Curr Genet (2016) 62: 887. doi:10.1007/s00294-016-0598-y

Abstract

We sequenced the complete plastid and mitochondrial genomes of the unicellular marine phytoplankton Triparma laevis, belonging to the order Parmales (Heterokonta). The cells of Parmales are surrounded by silicified cell walls, similar to Bacillariophyta (diatoms). T. laevis was recognized as a sister group of Bacillariophyta using a molecular phylogenetic analysis based on SSU rDNA and rbcL sequences. Bacillariophyta are the most successful group of phytoplankton in the modern ocean, but the origin and early evolution of them have not been clearly established. Detailed molecular analyses of T. laevis may increase our understanding of the evolutionary relationships among Parmales and Bacillariophyta. The gene contents of the plastid and mitochondrial genomes are similar between T. laevis and Bacillariophyta. The gene order of the plastid genome is also similar to Bacillariophyta, whereas the gene order of the mitochondrial genome is not conserved in Bacillariophyta, but the structure is more compact than Bacillariophyta. Phylogenetic analyses, using plastid-encoded concatenated amino acid datasets and mitochondria-encoded concatenated amino acid datasets suggest that T. laevis is a sister group of Bacillariophyta. These results suggest that the characteristics of the organellar genomes of T. laevis are similar and conserve ancestral characteristics more than Bacillariophyta.

Keywords

Genome sequencing Heterokonta Mitochondrial genome Parmales Plastid genome Triparma laevis 

Supplementary material

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Funding information

Funder NameGrant NumberFunding Note
the Global COE Program, ”From the Earth to ”Earths””
    the Canon Foundation
      the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
      • 26291085

      Copyright information

      © Springer-Verlag Berlin Heidelberg 2016

      Authors and Affiliations

      • Naoyuki Tajima
        • 1
      • Kenji Saitoh
        • 2
      • Shusei Sato
        • 3
      • Fumito Maruyama
        • 4
      • Mutsuo Ichinomiya
        • 5
      • Shinya Yoshikawa
        • 6
      • Ken Kurokawa
        • 7
      • Hiroyuki Ohta
        • 8
      • Satoshi Tabata
        • 9
      • Akira Kuwata
        • 10
      • Naoki Sato
        • 1
      1. 1.Department of Life Sciences, Graduate School of Arts and SciencesUniversity of TokyoMeguro-kuJapan
      2. 2.National Research Institute of Fisheries ScienceJapan Fisheries Research and Education AgencyYokohamaJapan
      3. 3.Department of Environmental Life Sciences, Graduate School of Life SciencesTohoku UniversitySendaiJapan
      4. 4.Graduate School of Medicine and Faculty of Medicine Kyoto University, Section of MicrobiologyKyotoJapan
      5. 5.Faculty of Environmental and Symbiotic SciencesPrefectural University of KumamotoKumamotoJapan
      6. 6.Faculty of Marine BioscienceFukui Prefectural UniversityObamaJapan
      7. 7.Department of Biological InformationTokyo Institute of TechnologyYokohamaJapan
      8. 8.Center for Biological Resources and InformaticsTokyo Institute of TechnologyYokohamaJapan
      9. 9.Kazusa DNA Research InstituteKisarazuJapan
      10. 10.Tohoku National Fisheries Research InstituteJapan Fisheries Research and Education AgencyShiogamaJapan

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