Current Genetics

, Volume 60, Issue 1, pp 49–59

Mitochondrial genome of the basidiomycetous yeast Jaminaea angkorensis

  • Eva Hegedusova
  • Brona Brejova
  • Lubomir Tomaska
  • Matthias Sipiczki
  • Jozef Nosek
Original Article

DOI: 10.1007/s00294-013-0410-1

Cite this article as:
Hegedusova, E., Brejova, B., Tomaska, L. et al. Curr Genet (2014) 60: 49. doi:10.1007/s00294-013-0410-1

Abstract

Jaminaea angkorensis is an anamorphic basidiomycetous yeast species originally isolated from decaying leaves in Cambodia. Taxonomically, J. angkorensis is affiliated with Microstromatales (Exobasidiomycetes, Ustilaginomycotina, Basidiomycota) and represents a basal phylogenetic lineage of this fungal order. To perform a comparative analysis of J. angkorensis with other basidiomycetes, we determined and analyzed its complete mitochondrial DNA sequence. The mitochondrial genome is represented by 29,999 base pairs long, circular DNA containing 32 % guanine and cytosine residues. Its genetic organization is relatively compact and comprises typical genes for 15 conserved proteins involved in oxidative phosphorylation (atp6, 8, and 9; cob; cox1, 2, and 3; and nad1, 2, 3, 4, 4L, 5, and 6) and translation (rps3), two ribosomal RNAs (rnl and rns) and twenty-two transfer RNAs (trnA-Y). Although the gene content is similar to other basidiomycetes, the gene orders in the examined species exhibit only a limited synteny, reflecting their phylogenetic distances and extensive genome rearrangements. In addition, a comparative analysis of basidiomycete mitochondrial genomes indicates that stop-to-tryptophan reassignment of the UGA codon was accompanied by structural alterations of tRNA-Trp(CCA). These results provide an insight into the evolution of the genetic code in fungal mitochondria.

Keywords

Mitochondrial DNA Codon usage tRNA-Trp Molecular phylogeny Jaminaea angkorensis Basidiomycota 

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Eva Hegedusova
    • 1
  • Brona Brejova
    • 2
  • Lubomir Tomaska
    • 3
  • Matthias Sipiczki
    • 4
  • Jozef Nosek
    • 1
  1. 1.Department of Biochemistry, Faculty of Natural SciencesComenius UniversityBratislavaSlovak Republic
  2. 2.Department of Computer Science, Faculty of Mathematics, Physics and InformaticsComenius UniversityBratislavaSlovak Republic
  3. 3.Department of Genetics, Faculty of Natural SciencesComenius UniversityBratislavaSlovak Republic
  4. 4.Department of Genetics and Applied MicrobiologyUniversity of DebrecenDebrecenHungary

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