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Transformation and electrophoretic karyotyping of Coniochaeta ligniaria NRRL30616

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Abstract

Coniochaeta ligniaria NRRL30616 is an ascomycete that grows with yeast-like appearance in liquid culture. The strain has potential utility for conversion of fibrous biomass to fuels or chemicals. Furans and other inhibitory compounds in lignocellulosic biomass are metabolized by NRRL30616, facilitating subsequent microbial fermentation of biomass sugars. This study undertook initial characterization of the genetic system of C.ligniaria NRRL30616. Transformation using hygromycin as a dominant selectable marker was achieved using protoplasts generated by incubating cells in 1% (v/v) β-mercaptoethanol, followed by cell wall-digesting enzymes. Thirteen chromosomes with an estimated total size of 30.1 Mb were detected in C. ligniaria. The GC content of chromosomal DNA and of coding regions from cDNA sequences were 49.2 and 51.9%, respectively. This study is the first report of genome size, electrophoretic karyotype, and transformation system for a member of the Coniochaetales.

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Acknowledgments

We thank Sarah E. Frazer, Kristina M. Glenzinski, and Rhonda L. Zeltwanger for their technical assistance.

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Authors and Affiliations

  1. USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N. University Street, Peoria, IL, 61604, USA

    Nancy N. Nichols & Christopher D. Skory

  2. School of Biological Sciences, Illinois State University, Normal, IL, USA

    Matthew P. Szynkarek & Wade A. Nichols

  3. Department of Biology, Central Michigan University, Mount Pleasant, MI, USA

    Steven W. Gorsich

  4. Departamento de Biología Aplicada, University of Almeria, Almeria, Spain

    Maria J. López & Gema M. Guisado

Authors
  1. Nancy N. Nichols
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  2. Matthew P. Szynkarek
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  3. Christopher D. Skory
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  4. Steven W. Gorsich
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  5. Maria J. López
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  6. Gema M. Guisado
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  7. Wade A. Nichols
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Corresponding author

Correspondence to Nancy N. Nichols.

Additional information

Communicated by H. Osiewacz.

Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession numbers HQ197357 and HS107146-HS107191.

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Nichols, N.N., Szynkarek, M.P., Skory, C.D. et al. Transformation and electrophoretic karyotyping of Coniochaeta ligniaria NRRL30616. Curr Genet 57, 169–175 (2011). https://doi.org/10.1007/s00294-010-0332-0

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  • DOI: https://doi.org/10.1007/s00294-010-0332-0

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