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An autophagy gene, MgATG5, is required for cell differentiation and pathogenesis in Magnaporthe oryzae

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Abstract

Autophagy is a conserved degradation pathway that is involved in the maintenance of normal cell differentiation and development. The Saccharomyces cerevisiae ATG5 gene is an important component of the autophagy process. In this study, we identified MgATG5 as an autophagy-related gene in Magnaporthe oryzae that is homologous to ATG5. Using targeted gene replacement, an Mgatg5∆ mutant was generated and fungal autophagy was blocked. Cytological analysis revealed that the mutant had poor fungal morphogenic development, including a shortened aerial hyphae lifespan, decreased conidiation and perithecia formation, delayed conidial germination and appressorial formation, postponement of conidial cytoplasm transfer during appressorium formation, and reduction in formation of the penetration peg. Turnover of endogenous matter in the Mgatg5∆ mutant was also affected, as demonstrated by defects in the formation of conidial lipid droplets, and in the degradation of conidial glycogen deposits during appressorium formation. Lipid droplets and glycogen are necessary to generate adequate turgor in appressoria for invading the host surface. As a result of the decreased appressorium turgor and differentiation in the penetration peg, Mgatg5∆ pathogenicity was deficient in two host plants tested. The developmental and pathogenic phenotypes were restored by the introduction of an intact copy of MgATG5 into Mgatg5∆, demonstrating that the MgATG5 deletion was responsible for the cellular defects. Taken together, these findings suggest that autophagy promotes cell differentiation through turnover of endogenous matter during fungal development, and is thus essential for the pathogenicity of the rice blast fungus.

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Acknowledgments

This study was supported by grants (No. 30470064, 30671351 and 30870101) from National Natural Science Foundation of China, a grant (No. Y304211) from Natural Science Foundation of Zhejiang province, a doctoral fund from Ministry of Education of China (No. 200803350086), a public project from Ministry of Agriculture of China (No. 200803008) and a major project (No. 2004C12020-5) from Zhejiang Science and Technology Bureau of China to F.-C. Lin.

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Yuhangtang Road 388, 310058, Hangzhou, China

    Jian-Ping Lu, Xiao-Xiao Feng & Hang Min

  2. Key State Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Kaixuan Road 268, 310029, Hangzhou, China

    Xiao-Hong Liu & Fu-Cheng Lin

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  1. Jian-Ping Lu
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Correspondence to Fu-Cheng Lin.

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Communicated by D. Ebbole.

J.-P. Lu and X.-H. Liu contributed equally to this work and are regarded joint first authors.

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Lu, JP., Liu, XH., Feng, XX. et al. An autophagy gene, MgATG5, is required for cell differentiation and pathogenesis in Magnaporthe oryzae . Curr Genet 55, 461–473 (2009). https://doi.org/10.1007/s00294-009-0259-5

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