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Mating-type loci of heterothallic Diaporthe spp.: homologous genes are present in opposite mating-types

Abstract

Sexual reproduction of fungi is governed by genes located on the mating-type (MAT) locus. To analyze the MAT locus of the genus Diaporthe (anamorph: Phomopsis), a large genera within the ascomycetous class Sordariomycetes, we cloned and sequenced loci MAT1-1 and MAT1-2 from two heterothallic Diaporthe species, designated as Diaporthe W- and G-types (four isolates in total). The mating-type loci structures of Diaporthe W- and G-types were similar; MAT1-1 isolates had a MAT locus containing three genes, MAT1-1-1, MAT1-1-2 and MAT1-1-3, as was the case with other Sordariomycetes, and in contrast to other Sordariomycetes, MAT1-2 isolates had genes homologous to MAT1-1-2 and MAT1-1-3, in addition to MAT1-2-1. Expression analysis by RT-PCR revealed that all the mating-type genes of Diaporthe W-type were transcriptionally active during vegetative growth. The structure of MAT loci of Diaporthe W- and G-types is distinct from that in other heterothallic filamentous ascomycetes, which have dissimilar gene structure in opposite mating-type loci. This unique structure is informative to discussing the evolutionary history and function of mating-type genes of Sordariomycete fungi.

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Acknowledgments

We are grateful to Dr. N. Matsumoto for critical review of this manuscript, and Dr. T. Arie for poviding the primers NcHMG1 and NcHMG2. This study was supported in part by a Grant-in-Aid (Bio Design Program) from the Ministry of Agriculture, Forestry and Fisheries of Japan.

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Correspondence to Satoko Kanematsu.

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Communicated by U. Kück.

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Kanematsu, S., Adachi, Y. & Ito, T. Mating-type loci of heterothallic Diaporthe spp.: homologous genes are present in opposite mating-types. Curr Genet 52, 11–22 (2007). https://doi.org/10.1007/s00294-007-0132-3

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  • DOI: https://doi.org/10.1007/s00294-007-0132-3

Keywords

  • Pyrenomycetes
  • Mating-type gene organization
  • Evolution