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The SIT4 gene, which encodes protein phosphatase 2A, is required for telomere function in Saccharomyces cerevisiae

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Abstract

Life span and number of cell divisions in eukaryotes are limited. The accumulation of stress-associated damage due to ageing may cause irreversible cell cycle arrest, so-called “cellular senescence”. Although many genes have been implicated in determining life span, regulatory systems that counteract age-related stress have not yet been clarified. We examined senescence during a stress of Saccharomyces cerevisiae strains carrying disruptions in protein phosphatase (PPase)-encoding genes in order to identify the system counteracting senescence. Among these strains, short telomeres were found in the sit4 disruptant that lacks one form of protein phosphatase 2A (PP2A). Silencing ability in the subtelomeric region was impaired and hyperphosphorylation of Sir3 was also observed in this mutant. The sit4 mutant was found to have altered nucleoli and a life span as short as an sgs1 mutant. These observations suggest that the PP2A pathway regulates life span in yeast.

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Acknowledgements

We are grateful to Drs. Araki (National Institute of Genetics, Japan), Shirahige (Tokyo Institute of Technology), Lundblad (Baylor College of Medicine), A. Kikuchi (Nagoya University), Y. Kikuchi, Kubota, Ota and Yamaguchi (University of Tokyo) for providing plasmids and yeast strains and for stimulating discussion.

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Authors and Affiliations

  1. Department of Molecular Oncology, Cancer Research Institute, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-0934, Japan

    Naoyuki Hayashi, Takahiro Nomura & Seishi Murakami

  2. Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan

    Naoko Sakumoto, Yukio Mukai, Yoshinobu Kaneko & Satoshi Harashima

  3. Division of Nutrition Science, Kanazawa Gakuin College, Kanazawa, Ishikawa, 920-1392, Japan

    Takahiro Nomura

  4. Laboratory for Developmental Genomics, RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan

    Naoko Sakumoto

Authors
  1. Naoyuki Hayashi
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  2. Takahiro Nomura
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  3. Naoko Sakumoto
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  4. Yukio Mukai
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  5. Yoshinobu Kaneko
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  6. Satoshi Harashima
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  7. Seishi Murakami
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Correspondence to Naoyuki Hayashi.

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Communicated by S. Hohmann

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Hayashi, N., Nomura, T., Sakumoto, N. et al. The SIT4 gene, which encodes protein phosphatase 2A, is required for telomere function in Saccharomyces cerevisiae. Curr Genet 47, 359–367 (2005). https://doi.org/10.1007/s00294-005-0577-1

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  • DOI: https://doi.org/10.1007/s00294-005-0577-1

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