Abstract
Southern analysis with rpl5 and rps14 mtDNA gene probes of Solanum tuberosum, S. commersonii and a sample of somatic hybrids detected polymorphisms between parents and the appearance of a novel restriction fragment in various hybrids. In one of them, detailed mtDNA analyses revealed various configurations of the rpl5–rps14 region present at different stoichiometries. Multiple inter-parental recombination events across homologous sequences were assumed to have caused these rearrangements. Sequence similarity searches detected one sequence putatively involved in the recombination upstream of the rpl5 gene. The presence of a second recombinogenic sequence was inferred. We propose two models to explain the mechanism responsible for obtaining the different rpl5–rps14 arrangements shown after somatic hybridization. Variability in the rpl5–rps14 region observed in both the parental species and their somatic hybrids suggests this region is a hot spot for mtDNA rearrangements in Solanum spp.
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Akagi H, Shimada H, Fujimura T (1995) High-frequency inter-parental recombination between mitochondrial genomes of rice cybrids. Curr Genet 29:58–65
Arrieta-Montiel M, Lyznik A, Woloszynska M, Janska H, Tohme J, Mackenzie S (2001) Tracing evolutionary and developmental implications of mitochondrial stoichiometric shifting in the common bean. Genetics 158:851–864
Bellaoui M, Martin-Canadell A, Pelletier G, Budar F (1998) Low-copy-number molecules are produced by recombination, actively maintained and can be amplified in the mitochondrial genome of Brassicaceae: relationship to reversion of the male sterile phenotype in some cybrids. Mol Gen Genet 257:177–185
Brandt P, Unseld M, Eckert-Ossenkoop U, Brennicke A (1993) An rps14 pseudogene is transcribed and edited in Arabidpsis mitochondria. Curr Genet 24:330–336
Cardi T, Bastia T, Monti L, Earle ED (1999) Organelle DNA and male fertility variation in Solanum spp. and interspecific somatic hybrids. Theor Appl Genet 99:819–828
Earle ED (1995) Mitochondrial DNA in somatic hybrids and cybrids. In: Levings CS III, Vasil I (eds) The molecular biology of plant mitochondria. Kluwer, Dordrecht, pp 557–584
Hartmann C, et al (1994) Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat. Curr Genet 25:456–464
Hoffmann M, Dombrowski S, Guha C, Binder S (1999) Cotranscription of the rpl5–rps14–cob gene cluster in pea mitochondria. Mol Gen Genet 261:537–545
Levings CS, Brown GG (1989) Molecular biology of plant mitochondria. Cell 56:171–179
Lonsdale DM, Brears T, Hodge TB, Melville SE, Rottmann WH (1988) The plant mitochondrial genome: homologous recombination as a mechanism for generating heterogeneity. Philos Trans R Soc Lond B Biol Sci 319:149–163
Lössl A, Adler N, Horn R, Frei U, Wenzel G (1999) Chondriome type characterization of potato: mt α, β, γ, δ, ε and novel plastid-mitochondrial configurations in somatic hybrids. Theor Appl Genet 99:1–10
Mackenzie S, McIntosh L (1999) Higher plant mitochondria. Plant Cell 11:571–585
Newton KJ (1988) Plant mitochondrial genomes: organization, expression and variation. Annu Rev Plant Physiol Plant Mol Biol 39:503–532
Quiñones V, et al (1996) The rpl5–rps14–cob arrangement in Solanum tuberosum: rps14 is a transcribed and unedited pseudogene. Plant Mol Biol 31:937–943
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Scotti N (2002) Variabilità naturale ed indotta nei genomi citoplasmatici di Solanum spp. ed interazioni nucleo-citoplasmatiche. PhD thesis. University “Federico II”, Naples
Scotti N, Cardi T, Maréchal-Drouard L (2001) Mitochondrial DNA and RNA isolation from small amounts of different potato tissues. Plant Mol Biol Rep 19:67a–67h
Scotti N, Monti L, Cardi T (2003) Organelle DNA variation in parental Solanum spp. genotypes and nuclear-cytoplasmic interactions in Solanum tuberosum (+) S. commersonii somatic hybrid-backcross progeny. Theor Appl Genet 108:87–94
Small ID, Isaac PG, Leaver CJ (1987) Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of the mitochondrial genome diversity in maize. EMBO J 6:865–869
Small ID, Suffolk R, Leaver CJ (1989) Evolution of plant mitochondrial genomes via substoichiometric intermediates. Cell 58:69–76
Stupar RM, et al (2001) Complex mtDNA constitutes an approximate 620-kb insertion on Arabidopsis thaliana chromosome 2: implication of potential sequencing errors caused by large-unit repeats. Proc Natl Acad Sci USA 98:5099–5103
Temple M, Makaroff CA, Mutschler MA, Earle ED (1992) Novel mitochondrial genomes in Brassica napus somatic hybrids. Curr Genet 22:243–249
Vedel F, Chétrit P, Mathieu C, Pelletier G, Primard C (1986) Several different mitochondrial DNA regions are involved in intergenomic recombination in Brassica napus cybrid plants. Curr Genet 11:17–24
Woloszynska M, Kieleczawa J, Ornatowska M, Wozniak M, Janska H (2001) The origin and maintenance of the small repeat in the bean mitochondrial genome. Mol Genet Genomics 265:865–872
Ye F, Bernhardt J, Abel WO (1993) Genes for ribosomal proteins S3, L16, L5, and S14 are clustered in the mitochondrial genome of Brassica napus L. Curr Genet 24:323–329
Acknowledgements
This work was partially funded by the CNR-CNRS Bilateral Agreement and MiPAF “Potato Breeding” Projects. An EMBO Short Term Fellowship (ASTF 9251) was awarded to N.S. We thank Dr. W. Craig for reviewing the English version of the manuscript.
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Contribution no. 39 from the Institute of Plant Genetics, Research Division of Portici.
Communicated by A. Brennicke
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Scotti, N., Maréchal-Drouard, L. & Cardi, T. The rpl5–rps14 mitochondrial region: a hot spot for DNA rearrangements in Solanum spp. somatic hybrids. Curr Genet 45, 378–382 (2004). https://doi.org/10.1007/s00294-004-0496-6
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DOI: https://doi.org/10.1007/s00294-004-0496-6