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Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae

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Abstract.

A 1.2-Mb DNA band from an isolate of Magnaporthe oryzae was detected in a pulsed-field gel. A chromosomal entity corresponding to this band was observed at the mitotic metaphase stage. This minichromosome, carrying many transposable elements and two telomeres, was transmitted to ascosporic F1 cultures in a non-Mendelian manner with frequent changes in its size and number. Segregation analysis with RFLP markers indicated that the minichromosome underwent structural rearrangements, such as deletion and duplication, not only during meiosis but also after meiosis. An ectopic sister chromatid recombination may cause the size variation of the minichromosomes.

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References

  • Akamatsu H, Taga M, Kodama M, Johnson R, Otani H, Kohmoto K (1999) Molecular karyotypes for Alternaria plant pathogens known to produce host-specific toxins. Curr Genet 35:647–656

    Article  CAS  PubMed  Google Scholar 

  • Butler DK, Metzenberg RL (1990) Expansion and contraction of the nucleolus organizer region of Neurospora: changes originate in both proximal and distal segments. Genetics 126:325–333

    CAS  PubMed  Google Scholar 

  • Couch BC, Kohn LM (2002) A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea. Mycologia 94:683–693

    CAS  Google Scholar 

  • Covert SF (1998) Supernumerary chromosomes in filamentous fungi. Curr Genet 33:311–319

    CAS  PubMed  Google Scholar 

  • Enkerli J, Garima B, Covert SF (1997) Nht1, a transposable element cloned from a dispensable chromosome in Nectria haematococca. Mol Plant-Microbe Interact 10:742–749

    CAS  Google Scholar 

  • Enkerli J, Reed H, Briley A, Bhatt G, Covert SF (2000) Physical map of a conditionally dispensable chromosome in Nectria haematococca mating population VI and location of chromosome breakpoints. Genetics 155:1083–1094

    CAS  PubMed  Google Scholar 

  • Eto Y, Ikeda K, Chuma I, Kataoka T, Kuroda S, Kikuchi N, Don LD, Kusaba M, Nakayashiki H, Tosa Y, Mayama S (2001) Comparative analysis of the distribution of various transposable elements in Pyricularia and their activity during and after the sexual cycle. Mol Gen Genet 264:565–577

    Article  CAS  PubMed  Google Scholar 

  • Farman ML, Leong SA (1995) Genetic and physical mapping of telomeres in the rice blast fungus, Magnaporthe grisea. Genetics 140:479–492

    CAS  PubMed  Google Scholar 

  • Farman ML Tosa Y, Nitta N, Leong SA (1996a) MAGGY, a retrotransposon in the genome of the rice blast fungus Magnaporthe grisea. Mol Gen Genet 251:665–674

    Article  CAS  PubMed  Google Scholar 

  • Farman ML, Taura S, Leong SA (1996b) The Magnaporthe grisea DNA fingerprinting probe MGR586 contains the 3′ end of an inverted repeat transposon. Mol Gen Genet 251:675–681

    Article  CAS  PubMed  Google Scholar 

  • Francis DM, Michelmore RW (1993) Two classes of chromosome-sized molecules are present in Bremia lactucae. Exp Mycol 17:284–300

    Article  CAS  Google Scholar 

  • Hamer JE, Farrall L, Orbach MJ, Valent B, Chumley FG (1989) Host species-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen. Proc Natl Acad Sci USA 86:9981–9985

    Google Scholar 

  • Kachroo P, Leong SA, Chattoo BB (1994) Pot2, an inverted repeat transposon from the rice blast fungus Magnaporthe grisea. Mol Gen Genet 245:339–348

    CAS  PubMed  Google Scholar 

  • Kachroo P, Leong SA, Chattoo BB (1995) Mg-SINE: a short interspersed nuclear element from the rice blast fungus, Magnaporthe grisea. Proc Natl Acad Sci USA 92:11125–11129

    Google Scholar 

  • Kachroo P, Ahuja M, Leong SA, Chattoo BB (1997) Organization and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea. Curr Genet 31:361–369

    Article  CAS  PubMed  Google Scholar 

  • Kato H, Yamamoto M, Yamaguchi-Ozaki T, Kadouchi H, Iwamoto Y, Nakayashiki H, Tosa Y, Mayama S, Mori N (2000) Pathogenicity, mating ability and DNA restriction fragment length polymorphisms of Pyricularia populations isolated from Gramineae, Bambusideae and Zingiberaceae plants. J Gen Plant Pathol 66:30–47

    CAS  Google Scholar 

  • Kim HG, Meinhardt LW, Benny U, Kistler HC (1995) Nrs1, a repetitive element linked to pisatin demethylase genes on a dispensable chromosome of Nectria haematococca. Mol Plant-Microbe Interact 8:524–531

    CAS  Google Scholar 

  • Klein HL (1995) Genetic control of intrachromosomal recombination. BioEssays 17:147–159

    CAS  PubMed  Google Scholar 

  • Leclair S, Ansan-Melayah D, Rouxel T, Balesdent MH (1996) Meiotic behaviour of the minichromosome in the phytopathogenic ascomycete Leptosphaeria maculans. Curr Genet 30:541–548

    Article  CAS  PubMed  Google Scholar 

  • Miao VP, Covert SF, VanEtten HD (1991) A fungal gene for antibiotic resistance on a dispensable ("B") chromosome. Science 254:1773–1776

    CAS  PubMed  Google Scholar 

  • Murakami J, Tosa Y, Kataoka T, Tomita R, Kawasaki J, Chuma I, Sesumi Y, Kusaba M, Nakayashiki H, Mayama S (2000) Analysis of host species specificity of Magnaporthe grisea toward wheat using a genetic cross between isolates from wheat and foxtail millet. Phytopathology 90:1060–1067

    Google Scholar 

  • Nagy R, Táborhegyi É, Wittner A, Hornok L (1995) Mini-chromosomes in Fusarium sporotrichioides are mosaics of dispersed repeats and unique sequences. Microbiology 141:713–719

    CAS  PubMed  Google Scholar 

  • Nakayashiki H, Kiyotomi K, Tosa Y, Mayama S (1999) Transposition of the retrotransposon MAGGY in heterologous species of filamentous fungi. Genetics 153:693–703

    CAS  PubMed  Google Scholar 

  • Nakayashiki H, Matsuo H, Chuma I, Ikeda K, Betsuyaku S, Kusaba M, Tosa Y, Mayama S (2001) Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains. Nucleic Acids Res 29:4106–4113

    Article  CAS  PubMed  Google Scholar 

  • Nitta N, Farman ML, Leong SA (1997) Genome organization of Magnaporthe grisea: integration of genetic maps, clustering of transposable elements and identification of genome duplications and rearrangements. Theor Appl Genet 95:20–32

    Article  CAS  Google Scholar 

  • Orbach MJ, Chumley FG, Valent B (1996) Electrophoretic karyotypes of Magnaporthe grisea pathogens of diverse grasses. Mol Plant-Microbe Interact 9:261–271

    CAS  Google Scholar 

  • Shiflett AM, Enkerli J, Covert SF (2002) Nht2, a copia LTR retrotransposon from a conditionally dispensable chromosome in Nectria haematococca. Curr Genet 41:99–106

    Article  CAS  PubMed  Google Scholar 

  • Taga M, Murata M (1994) Visualization of mitotic chromosomes in filamentous fungi by fluorescence staining and fluorescence in situ hybridization. Chromosoma 103:408–413

    Article  CAS  PubMed  Google Scholar 

  • Taga, M. Murata M, Saito H (1998) Comparison of different karyotyping methods in filamentous ascomycetes—a case study of Nectria haematococca. Mycol Res 102:1355–1364

    Article  CAS  Google Scholar 

  • Taga M, Murata M, VanEtten HD (1999) Visualization of a conditionally dispensable chromosome in the filamentous ascomycete Nectria haematococca by fluorescence in situ hybridization. Fungal Genet Biol 26:169–177

    Article  CAS  PubMed  Google Scholar 

  • Talbot NJ, Salch YP, Ma M, Hamer JE (1993) Karyotypic variation within clonal lineages of the rice blast fungus, Magnaporthe grisea. Appl Environ Microbiol 59:585–593

    CAS  Google Scholar 

  • Tosa Y, Nakayashiki H, Hyodo H, Mayama S, Kato H, Leong SA (1995) Distribution of retrotransposon MAGGY in Pyricularia species. Ann Phytopathol Soc Jpn 61:549–554

    CAS  Google Scholar 

  • Tsuchiya D, Taga M (2001) Cytological karyotyping of three Cochliobolus spp by the germ tube burst method. Phytopathology 91:354–360

    Google Scholar 

  • Tzeng T-H, Lyngholm LK, Ford CF, Bronson CR (1992) A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal pathogen Cochliobolus heterostrophus. Genetics 130:81–96

    CAS  PubMed  Google Scholar 

  • Urashima AS, Hashimoto Y, Don LD, Kusaba M, Tosa Y, Nakayashiki H, Mayama S (1999) Molecular analysis of the wheat blast population in Brazil with a homolog of retrotransposon MGR583. Ann Phytopathol Soc Jpn 65:429–436

    CAS  Google Scholar 

  • Valent B, Chumley FG (1991) Molecular genetic analysis of the rice blast fungus, Magnaporthe grisea. Annu Rev Phytopathol 29:443–467

    CAS  Google Scholar 

  • Xu J, Leslie F (1996) A genetic map of Gibberella fijikuroi mating population A (Fusarium moniliforme). Genetics 143:175–189

    CAS  PubMed  Google Scholar 

  • Zolan ME (1995) Chromosome-length polymorphism in fungi. Microbiol Rev 59:686–98

    CAS  PubMed  Google Scholar 

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Acknowledgements.

We thank Dr. S.A. Leong, University of Wisconsin–Madison, for providing the cosmid vector pMLF2 and the cosmid markers. Special thanks are due to Dr. H. Kato, former professor of Kobe University, for valuable suggestions and encouragement.

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Authors and Affiliations

  1. Faculty of Agriculture, Kobe University, 657-8501, Kobe, Japan

    I. Chuma, Y. Tosa, H. Nakayashiki & S. Mayama

  2. Department of Biology, Faculty of Science, Okayama University, Tsushima-naka, 700-8530, Okayama, Japan

    M. Taga

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  1. I. Chuma
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  2. Y. Tosa
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  3. M. Taga
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  4. H. Nakayashiki
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  5. S. Mayama
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Corresponding author

Correspondence to Y. Tosa.

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Communicated by U. Kück

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Chuma, I., Tosa, Y., Taga, M. et al. Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae . Curr Genet 43, 191–198 (2003). https://doi.org/10.1007/s00294-003-0390-7

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  • DOI: https://doi.org/10.1007/s00294-003-0390-7

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