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cDNA encoding protein O-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2

Abstract

O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT genes have been cloned coding for these enzymes. In the present work, the characterisation of the pmt1 cDNA from T. reesei is reported. Sequence analysis of the predicted protein revealed the highest similarity to Schizosaccharomyces pombe Pmt and to Pmt4p of Saccharomyces cerevisiae. In contrast, expression of the T. reesei cDNA in various S. cerevisiae pmt mutants showed functional similarity to the yeast Pmt2 protein.

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Acknowledgements

We thank Prof. Widmar Tanner and Dr. Thomas Aust for chitinase and Gas1p antibodies and Dr. Sabine Strahl for the S. cerevisiae Δpmt strains. We also thank Prof. Francis Karst for the yeast expression plasmids. This work was supported by the State Committee for Scientific Research (KBN), Warsaw, Poland, project no. 6P04B00621 grant to J.S.K.

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Correspondence to Joanna S. Kruszewska.

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Communicated by S. Hohmann

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Zakrzewska, A., Migdalski, A., Saloheimo, M. et al. cDNA encoding protein O-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2 . Curr Genet 43, 11–16 (2003). https://doi.org/10.1007/s00294-003-0368-5

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Keywords

  • pmt gene
  • Protein glycosylation
  • O-Mannosylation
  • Secretion