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Präkanzerosen des Urothels

Von der Feulgen-Färbung bis zur Einzelzell-CGH

Precancerous lesions of the urothelium

From Feulgen staining to single cell CGH

  • Schwerpunkt: Uropathologie
  • Published:
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Zusammenfassung

Die Feulgen-Färbung mit der Möglichkeit der quantitativen Wiedergabe der DNA-Menge eines Kerns ist eine gute und einfache Methode, die unregelmäßige DNA-Vermehrung einer malignen Zelle wiederzugeben, welche wiederum Ausdruck der genetischen Instabilität ist. Die genetische Instabilität einer Tumorzelle ist das Kernstück der WHO-Klassifikation des Harnblasenkarzinoms aus dem Jahr 2004, innerhalb derer flache und papilläre Neoplasien in „Low-“ und „High-grade-Läsionen“ eingeteilt werden. „High grade“ gibt die genetische Instabilität und die damit verbundene höhere Progressionswahrscheinlichkeit wieder. Begleitende konkrete genetische Alterationen über die rein numerischen hinaus werden zunehmend diskriminierend identifiziert und zugeordnet. Der Stand der genetischen Untersuchungen insbesondere bei den Präkanzerosen wird hier im Kontext mit Morphologie (histologischer und zytologischer Diagnostik) und Klinik schwerpunktmäßig zusammengefasst.

Abstract

Feulgen staining represents a staining method to quantitatively document the DNA content of a nucleus. Thus it is an excellent and straightforward method to reflect the irregular increase in DNA content of a malignant cell as a sign of genetic instability. Genetic instability of the tumour cell is the key feature of the 2004 WHO classification of bladder tumours, in which flat and papillary neoplasia are grouped into low- and high-grade lesions. “High grade” represents the tumor with genetic instability and consequently a higher likelihood of progression. Concomitant distinct genetic aberrations other than the numeric ones are increasingly identified as discriminators and help group the entities. The current status of genetic investigations, especially those in precancerous lesions, will be outlined in this review in the context of morphology (histology and cytology) as well as clinical situation.

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Danksagung

Die hier geschilderten Arbeiten wurden z. T. mit Forschungsgeldern der DFG gefördert: KN263 9–2, Sp 460/5–2.

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Authors and Affiliations

  1. Institut für Pathologie, Universitätsklinik RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Deutschland

    R. Knüchel-Clarke, S.V. Koufou & K. Schwamborn

  2. Institut für Humangenetik, Universität Graz, Graz, Österreich

    M. Speicher

  3. Urologische Klinik und Poliklinik, Ludwig-Maximilian-Universität München, München, Deutschland

    D. Zaak

  4. Institut für Pathologie, Universitätsklinik Erlangen, Erlangen, Deutschland

    R. Stöhr

Authors
  1. R. Knüchel-Clarke
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  2. S.V. Koufou
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  3. M. Speicher
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  4. K. Schwamborn
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  5. D. Zaak
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  6. R. Stöhr
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Correspondence to R. Knüchel-Clarke.

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Knüchel-Clarke, R., Koufou, S., Speicher, M. et al. Präkanzerosen des Urothels. Pathologe 29, 364–370 (2008). https://doi.org/10.1007/s00292-008-1015-1

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  • DOI: https://doi.org/10.1007/s00292-008-1015-1

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