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Quantitative methylierungsspezifische PCR zur Lungenkarzinomdiagnostik

Quantitative methylation-specific PCR for the diagnosis of lung cancer

  • Schwerpunkt: Zytopathologie
  • Published:
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Zusammenfassung

Die rasche, möglichst frühe Diagnose des Lungenkarzinoms stellt eine große Herausforderung dar. Die Sensitivität der konventionellen histo- und zytomorphologischen Verfahren ist diesbezüglich unbefriedigend. Der vorliegende Beitrag beleuchtet Technik, Chancen und Probleme des Nachweises aberranter Promotormethylierungen als Biomarker für die Lungenkarzinomdiagnostik an Materialien der pulmonalen Exfoliativzytologie.

Abstract

Efficient, preferably early diagnosis of lung cancer represents a major challenge. Under this aspect the sensitivity of conventional histomorphology and cytomorphology procedures is unsatisfactory. This review highlights technical aspects, possibilities and drawbacks of the application of aberrant promoter methylation as a biomarker for lung cancer diagnostics using specimens of pulmonary exfoliative cytology.

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Authors and Affiliations

  1. Target Research,Merck KGaA, Frankfurter Straße 250, 64293, Darmstadt, Deutschland

    H.J. Grote

  2. Institut für Cytopathologie, Universität Düsseldorf, Düsseldorf, Deutschland

    H.J. Grote, V. Schmiemann, M. Kazimirek & A. Böcking

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  1. H.J. Grote
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  2. V. Schmiemann
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  3. M. Kazimirek
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  4. A. Böcking
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Correspondence to H.J. Grote.

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Grote, H., Schmiemann, V., Kazimirek, M. et al. Quantitative methylierungsspezifische PCR zur Lungenkarzinomdiagnostik. Pathologe 28, 377–383 (2007). https://doi.org/10.1007/s00292-007-0929-3

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  • DOI: https://doi.org/10.1007/s00292-007-0929-3

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